从人造碱基重议天然碱基结构、性质与功能  

Rethink of the native base structures,base pairing,and functions through non-natural bases

作　　者：王志鹏 王田[3,4] 马新雨 张军 

机构地区：[1]德州农工大学化学系,德克萨斯州美国77840 [2]重庆市南开中学,重庆400030 [3]清华大学化学系,北京100084 [4]加州大学圣地亚哥分校生命科学系,加利福利亚州美国92093

出　　处：《生命的化学》2017年第6期946-951,共6页Chemistry of Life

摘　　要：自然界中,碱基的互补配对对DNA双螺旋的结构稳定与功能实现有重要作用,也是高校相关生物化学、分子生物学、化学生物学、生物有机化学等课程教学的重要内容。近年来随着人造碱基的逐渐发展,合成生物学极大地拓宽了碱基结构与互补配对的模式,同时也反映出教学中对于天然碱基互补配对的一些不当或不完善的认识。本文从最近的人造碱基配对入手,分析其设计本质和应用特性,并借此重新认识天然碱基配对的结构与性质,探讨传统教学中不当的论点,以期对教学与科研有助。The base pairing is of great importance to the stabilization of DNA native double helical structure, which is also a main instruction part for college courses such as biochemistry, molecular biology, chemical biology, bioorganic chemistry, etc. With the newly developed artificial base structures and base pairing modes recently, synthetic biology is largely extending our understanding of biological systems in nature, which however reflects some bias or misunderstanding existing in college education. This manuscript will start with the introduction of the recent artificial base pairs, followed by the analysis of their essences and properties. We hope through rethinking of the base structures and base pairing modes, we can improve our current education material and concept, which will further benefit to teaching and research.

关 键 词：非天然碱基 核酸 核苷酸 氢键 生物化学 

分 类 号：Q52-4[生物学—生物化学]