葡萄籽原花青素对自发性高血压大鼠肾损伤的保护作用  

Protective effect of grape seed procyanidins against kidney injury in spontaneously hypertensive rats

作　　者：王晓玲[1] 张先杰[1,2] 黄霜枝 陈燕玲 唐剑涛[3] 吴秀香[1] 

机构地区：[1]遵义医学院珠海校区病理生理学教研室,珠海519041 [2]绥阳县人民医院心内科,遵义563300 [3]遵义医学院珠海校区计算机教研室,珠海519041

出　　处：《中药药理与临床》2017年第5期43-47,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：贵州省科技计划项目(黔科合SY字(2012)3114);贵州省优秀科技教育人才省长专项资金项目(黔省专合字(2012)91)

摘　　要：目的:研究葡萄籽原花青素对自发性高血压大鼠(SHR)肾损伤的保护作用并对其机制进行探讨。方法:8周龄雄性自发性高血压大鼠24只随机分成4组,每组6只:模型组、葡萄籽原花青素50mg/kg剂量组和200mg/kg剂量组、卡托普利组(30mg/kg),6只8周龄雄性Wistar-Kyoto大鼠设为对照组。治疗6周后,测定大鼠尾动脉收缩压(SBP);ELISA法测定大鼠尿中视黄醛结合蛋白(RBP)和α1微球蛋白(α1-MG)含量;HE染色下用图像分析软件测定肾内小动脉中膜厚度与血管内径比值(MT/LD);Masson染色下观察肾脏纤维化情况,测量并计算肾小球胶原纤维沉积评分(GCDS)及肾小管间质病变评分(TIDS);Western blot法检测肾皮质中磷酸化细胞外信号调节激酶1/2(p-ERK1/2)蛋白表达情况。结果:葡萄籽原花青素50mg/kg和200mg/kg剂量组能显著降低自发性高血压大鼠尾动脉收缩压、尿液中RBP和α1-MG含量、肾内小动脉MT/LD,减少肾脏组织中胶原纤维含量、降低GCDS和TIDS以及肾皮质中p-ERK1/2蛋白表达,葡萄籽原花青素200mg/kg剂量组的作用尤为显著。结论:葡萄籽原花青素对自发性高血压大鼠肾脏损伤有减轻作用,其机制可能与葡萄籽原花青素降低尾动脉收缩压、减少肾皮质中p-ERK1/2蛋白表达有关。Objective： To study the protective effect of grape seed procyanidins（GSP） against kidney injury in spontaneously hypertensive rats（SHR） and to explore its possible mechanisms. Methods： 24 male SHRs（8-week-old） were randomly divided into the model group,low and high dose of GSP groups（50 and 200 mg/kg）,and captopril was used in the positive control group（30 mg/kg）. Meanwhile,6 Wistar Kyoto rats（8-week-old） were randomly served as the control group. After 6 weeks＇ treatment,the tail systolic pressure（SBP） was measured by tail cuff method. Retinol binding protein（RBP） and alpha 1 microglobulin（α1-MG） contents were examined by ELISA. MT/LD of renal small artery,the renal fibrosis,glomerular collagen deposition score（GCDS） and tubular interstitial disease score（TIDS） were observed or were calculated by HE staining,Masson staining and by morphometry. The expression of phosphorylated extracelluar signal-regulated kinase1/2（p-ERK1/2） in renal cortex was detected by western blot analysis. Results： Grape seed procyanidins can significantly decrease tail systolic pressure,the RBP and α1-MG contents of urine,MT/LD of renal small artery,the collagen accumulation in renal tissue,GCDS and TIDS,as well as the expression of p-ERK1/2 in renal cortex. These effects were more notable in high dose of grape seed procyanidin group（200 mg/kg）. Conclusion： Grape seed procyanidin can effectively protect SHR from the kidney injury by decreasing tail SBP and reducing the expression of p-ERK1/2 in renal cortex.

关 键 词：葡萄籽原花青素 自发性高血压大鼠 肾脏损伤 

分 类 号：R285.5[医药卫生—中药学]