补肾强督治偻方可改善地塞米松对大鼠成骨细胞的骨破坏作用  

Bushenqiangduzhilv Decoction can effectively improve the bone destruction of rat osteoblasts by dexamethasone in vitro

作　　者：邱冬妮[1] 林洁华[1] 徐永跃 谭希[1] 赖勇辉 何羿婷 

机构地区：[1]广州中医药大学,广州510403 [2]广州中医药大学第二附属医院,广州510120

出　　处：《中药药理与临床》2017年第6期105-109,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：2016年度国家自然基金资助项目(NO.81673898);2015年度广东省高水平大学中西医结合重点学科建设项目(NO.YN2015MJ06);2016年度国家中医药管理局国家中医临床研究基地业务建设科研专项(NO.JDZX2015201)**

摘　　要：目的:研究补肾强督治偻汤方对体外培养大鼠成骨细胞的作用。方法:以地塞米松为诱导剂,使用MTT法、对硝基苯磷酸盐法及茜素红染色法观察补肾强督治偻汤复方影响体外培养大鼠成骨细胞增殖、碱性磷酸酶表达及矿化结节形成的作用。结果:与地塞米松组相比,地塞米松+补肾强督治偻汤组(10^(-8)g/ml、2×10^(-8)g/ml、4×10^(-8)g/ml、8×10^(-8)g/ml)能促进大鼠成骨细胞的增殖表达,24h^48h时明显。与地塞米松组相比,地塞米松+补肾强督治偻汤组可以提高大鼠成骨细胞的碱性磷酸酶活性,第7天时,地塞米松+补肾强督治偻汤组(4×10^(-8)g/ml)有显著差异;第14天时,地塞米松+补肾强督治偻汤组(4×10^(-8)g/ml)有统计学意义。与地塞米松组相比,地塞米松+补肾强督治偻汤组(10^(-8)g/ml、2×10^(-8)g/ml、4×10^(-8)g/ml、8×10^(-8)g/ml)及补肾强督治偻汤组(10^(-8)g/ml、2×10^(-8)g/ml、4×10^(-8)g/ml、8×10^(-8)g/ml)均可以显著提高大鼠成骨细胞矿化结节形成的数量。结论:补肾强督治偻汤方有助于刺激体外培养大鼠成骨细胞增殖和分化成熟,可有效保护地塞米松对大鼠成骨细胞的骨破坏。Objective： To study the impact of bushenqiangduzhilv decoction extracted from traditional Chinese medicine on cultured osteoblasts in vitro. Methods： Dexamethasone was used as an inducer,the proliferation,activity of alkaline phosphatase enzyme and the number of mineral nodules of osteoblasts were measured by MTT,PNPP and ARS in vitro. Results： 1. Compared with dexamethasone group,dexamethasone ＋bushenqiangduzhilv decoction（ 10^-8 g/ml,2 × 10^-8 g/ml,4 × 10^-8 g/ml,8 × 10^-8 g/ml） promoted the proliferation of osteoblasts in rats,and it was obvious at 24 h ~ 48 h（ P〈0. 05 ~ 0. 01）. Compared with dexamethasone group,activities of alkaline phosphatase enzyme of osteoblasts of rats in dexamethasone ＋ bushenqiangduzhilv decoction（ 4 × 10^-8 g/ml） group were significantly high on the 7 th day（ P〈0. 05） and on the14 th day（ P〈0. 01）. Compared with dexamethasone group,bushenqiangduzhilv decoction（ 10^-8 g/ml,2 × 10^-8 g/ml,4 × 10^-8 g/ml,8 × 10^-8 g/ml） significantly increased the number of mineralized nodules（ P〈0. 01）. Conclusions： bushenqiangduzhilv decoction decoction can help to simulate the proliferation and differentiation of rat osteoblasts in vitro,which can effectively protect the bone damage of osteoblasts induced by low dose of dexamethasone.

关 键 词：补肾强督治偻方 细胞培养 成骨细胞 

分 类 号：R285.5[医药卫生—中药学]