霍山石斛内生真菌Alternaria eichhorniae次生代谢产物研究  

Study on Secondary Metabolites of Endophytic Fungus Alternaria eichhorniae from Dendrobium huoshanense

作　　者：王国凯 郁阳 刘劲松 张培良 马宗慧 梁益敏 王刚 

机构地区：[1]安徽中医药大学药学院/安徽道地中药材品质提升协同创新中心,安徽合肥230012

出　　处：《中药材》2017年第7期1614-1617,共4页Journal of Chinese Medicinal Materials

基　　金：国家中医药管理局"中药化学"重点学科建设项目;安徽省高校自然科学基金重点项目(KJ2016A856)

摘　　要：目的:研究霍山石斛内生真菌Alternaria eichhorniae的次生代谢产物。方法:利用硅胶柱层析、Sephadex LH-20、反相制备高效液相色谱等多种色谱法分离化合物,根据波谱方法对化合物进行结构鉴定。结果:从霍山石斛内生真菌Alternaria eichhorniae中分离得到12个化合物,分别鉴定为:tricycloalternarenes 6b(1)、tricycloalternarenes 2a(2)、tricycloalternarenes 1b(3)、tricycloalternarenes 1a(4)、tricycloalternarenes H(5)、stemphyperylenol(6)、altertoxinsⅠ(7)、alterperylenol(8)、(2R,3S)-1-(4-hydroxyphenyl)butane-2,3-diol(9)、3-羟基-4-(4-羟基苯基)-2-丁酮(10)、melithasterol B(11)、(22E,24R)-3β,5α,9α-trihydroxyergosta-7,22-dien-6-one(12)。结论:所有化合物均首次从霍山石斛内生真菌Alternaria eichhorniae中分离得到。Objective： To study the secondary metabolites of Endophytic fungus Alternaria eichhorniae from Dendrobium huoshanense.Methods： The compounds were isolated from the Et OAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis.Results： Twelve compounds were isolated and their structures were identified as tricycloalternarenes 6 b（1）,tricycloalternarenes 2 a（2）,tricycloalternarenes 1 b（3）,tricycloalternarenes 1 a（4）,tricycloalternarenes H（5）,stemphyperylenol（6）,altertoxins Ⅰ（7）,alterperylenol（8）,（2 R,3 S）-1-（4-hydroxyphenyl） butane-2,3-diol（9） 、3-hydroxy-4-（4-hydroxyphenyl）-2-butanone（10）,melithasterol B（11） and（22 E,24 R）-3β,5α,9α-trihydroxyergosta-7,22-dien-6-one（12）.Conclusion： All the compounds are isolated from Alternaria eichhorniae for the first time.

关 键 词：ALTERNARIA eichhorniae 内生真菌 霍山石斛 次生代谢产物 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]