异补骨脂素对光老化HDF细胞ER/TGF-β_1/Smads信号通路的调控效应研究  

Isopsoralen on Regulating Effect of HDF Cells ER/TGF-β_1/Smads Signaling Pathway of Photoaging

作　　者：顾婷[1] 徐占玲 朴成玉[1] 刘爽[1] 高海娜[1] 吴修红[1] 张宁[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中药材》2017年第7期1683-1686,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81274035);黑龙江省博士后科研启动基金(LBH-Q13162);黑龙江中医药大学优秀创新人才支持计划(2012)

摘　　要：目的:研究异补骨脂素对光老化人真皮成纤维(HDF)细胞ER/TGF-β_1/Smads信号通路的调控效应。方法:建立长波紫外线(UVA)损伤HDF模型,给予不同浓度异补骨脂素和通路阻断剂进行干预,MTT法检测细胞增殖率,Western blotting和Real Time-PCR法检测各组细胞内信号转导分子(Smad3)、转化生长因子(TGF-β_1)、Ⅰ型胶原(COL1A1)蛋白和对应mRNA的表达。结果:与正常对照组比较,模型组增殖率及Smad3、TGF-β_1、COL1A1蛋白和mRNA表达均显著降低(P<0.01);与模型组比较,各给药组增殖率和Smad3、TGF-β_1、COL1A1蛋白及mRNA表达均显著升高(P<0.01);与异补骨脂素高浓度组比较,TGF-β_1、Smad3、ER三个阻断剂组相对应的TGF-β_1、Smad3和COL1A1蛋白及相应mRNA表达均显著降低(P<0.01)。结论:异补骨脂素能增加HDF细胞TGF-β_1、Smad3、COL1A1蛋白及mRNA表达,对光老化HDF细胞胶原合成有促进作用;但这种作用能被TGF-β_1、Smad3和ER阻断剂所抑制,推测异补骨脂素促进胶原合成的作用机制与ER/TGF-β_1/Smads信号通路有关。Objective： To study the regulation effect of isopsoralen on HDF cells ER/TGF-β/Smads signaling pathway of photoaging.Methods： The UVA HDF injury model was established,given different doses of isopsoralen and pathway inhibitor,the proliferation rate of cells was measured by MTT assay.Western blotting and Real Time-PCR method were used to detect the protein expression of Smad3,TGF-β1,COL1A1 and the corresponding mRNA.Results： Compared with the control group,the proliferation rate of cells,and Smad3,TGF-β1,COL1A1 protein expression and mRNA expression were significantly reduced（P 0.01）; compared with the model group,cells proliferation rate,Smad3,TGF-β1,COL1A1 protein expression and mRNA expression of different drug groups were significantly increased（P 0.01）.Compared with the high dose group of isopsoralen,three blockers TGF-β1,Smad3,ER corresponding to TGF-β1,Smad3 and COL1A1 protein expression and the corresponding mRNA expression were significantly decreased（P 0.01）.Conclusion： Isopsoralen can increase HDF cells TGF-β1,Smad3,COL1A1 protein expression and the corresponding mRNA expression,and promote light aging HDF collagen synthesis; but the effect can be blocked by TGF-β1,Smad3 and ER by the blockade,the mechanism of the promotion collagen synthesis may be related to the ER/TGF-β/Smads signal pathway.

关 键 词：异补骨脂素 光老化HDF细胞 ER/TGF-β1/Smads信号通路 

分 类 号：R285.5[医药卫生—中药学]