外源AgNO_3对秦艽幼苗生长及龙胆苦苷生物合成的影响  

Effect of Exogenous AgNO_3 on the Growth and Gentiopicroside Accumulation of Gentiana macrophylla Seedlings

作　　者：杨新兵 郭晓荣 王怀琴 周中枢 曹晓燕[1] 

机构地区：[1]陕西师范大学/西北濒危药材资源开发国家工程实验室/药用资源与天然药物化学教育部重点实验室,陕西西安710062

出　　处：《中药材》2017年第8期1758-1762,共5页Journal of Chinese Medicinal Materials

基　　金：陕西省自然科学基金(2014JQ3107);中央高校基本科研业务费专项资金项目(GK201302043);陕西师范大学大学生创新创业训练计划项目(CX16029)

摘　　要：目的:探究外源AgNO_3对秦艽幼苗生长和龙胆苦苷含量积累的影响。方法:以秦艽Gentiana macrophylla Pall.幼苗为研究对象,利用高效液相色谱法检测外源施加AgNO_3后秦艽幼苗中龙胆苦苷含量的积累变化及其对秦艽幼苗生长的影响,进一步利用实时荧光定量PCR技术检测龙胆苦苷生物合成途径上多个酶基因的表达。结果:当秦艽幼苗在含有1 mmol/L AgNO_3的MS培养基上生长9 d时,秦艽幼苗中龙胆苦苷的含量是对照的1.91倍,鲜重、干重以及根长平均增加了29%、32%和43%;荧光定量PCR结果显示,龙胆苦苷合成途径上多个酶基因的表达显著上调。结论:外源AgNO_3可显著促进秦艽幼苗中龙胆苦苷的积累,同时可显著促进秦艽幼苗生物量(干重、鲜重)的增加和根的伸长。Objective： To investigate the effects of exogenous AgNO_3 on the growth and gentiopicroside accumulation of Gentiana macrophylla seedlings. Methods： Gentiana macrophylla seedlings were used to reserch,the concentrations of gentiopicroside in Gentiana macrophylla seedlings treated with 1 mmol/L AgNO_3 were measured by HPLC method. The growth of the Gentiana macrophylla seedlings were observed after treated with AgNO_3. The expression of key enzyme geneson and the gentiopicroside biosynthesis pathway were detected by Real time fluorescent quantitative PCR（ RT-q PCR）. Results： Compared with control group,the content of gentiopicroside in Gentiana macrophylla seedlings increased to 1. 91 times,and the fresh weight,dry weight and root length were increased by an average of 29%,32% and 43% when they were treated with 1 mmol/L AgNO_3 for 9 d. RT-q PCR results showed that the expression of some key enzyme geneson gentiopicroside biosynthesis pathway were significantly enhanced after treated with exogenous AgNO_3. Conclusion： Exogenous AgNO_3 can significantly promote the accumulation of gentiopicroside in Gentiana macrophylla seedlings,increase the biomass（ dry weight and fresh weight） and root elongation of Gentiana macrophylla seedling.

关 键 词：秦艽 AGNO3 龙胆苦苷 RT-QPCR 

分 类 号：R282.2[医药卫生—中药学]