木香根腐病病原菌鉴定及生物学特性研究  

The Primary Studies on Pathogen Identification and Biological Characteristics of Saussurea lappa

作　　者：韩凤[1,2] 李巧玲 余中莲 林茂祥 杨成前[1,2] 韩如刚[1] 刘杰 

机构地区：[1]重庆市药物种植研究所,重庆南川408435 [2]重庆市中药良种选育与评价工程技术中心,重庆南川408435

出　　处：《中药材》2017年第5期1020-1025,共6页Journal of Chinese Medicinal Materials

基　　金：重庆市卫生局中医药科技项目(KJ20130161);重庆市南川区科委项目(cx201509);重庆市科委基本科研业务费项目(2016cstcjbky-01304)

摘　　要：目的:分离和鉴定木香根腐病的病原菌,探讨不同培养基、碳氮源、温度、pH等条件对根腐病病原菌产孢和菌丝生长的影响,以期掌握生物学特性,为该病的田间防治提供理论依据。方法:通过病原菌形态学观察,结合rDNA-ITS测序,在Genbank中经Blast搜索相似性序列,以ITS序列经MEGA 6.0程序构建其分子系统发育树,确定引起木香根腐病的病原菌15b为镰刀菌属真菌Fusarium oxysporum f.sp.ciceris isolate。结果:菌株15b最适菌丝生长的培养基为PDA,生长最适碳、氮源分别为可溶性淀粉和蛋白胨,最适温度为25℃,最适pH值为6;产孢最适碳、氮源分别为可溶性淀粉和蛋白胨,最适温度为30℃,最适pH值为7;光照对菌丝生长发育没有明显影响,但24 h黑暗条件有利于促进孢子产生;菌丝致死温度为61℃、10 min。结论:Fusarium oxysporum f.sp.ciceris isolate是引起木香根腐病的致病菌。Objective： To isolate and identify pathogen of Saussurea lappa root-rot disesse,to study the effects of culture,carbon,nitrogen,temperature,pH on the sporulation and hyphal growth of pathogens,and to research the primary biological features of the fungi to offer a theoretical foundation for the prevention of this disease in the field.Methods： By observing the morphological characters of pathogens and rDNA-ITS sequencing,searching similar sequences by Blast in Genbank,the molecular phylogenetic tree was constructed by MEGA 6.0 program in ITS sequence,and the pathogen 15b was identified as Fusarium oxysporum f.sp.ciceris isolate.Results： Biological characteristics measurement showed that PDA was the optimal medium for mycelium growth,the optimum carbon and nitrogen sources were soluble starch and peptone,the optimal temperature was 25 ℃,the optimal pH value was 6.The optimum carbon and nitrogen sources of sporulation were soluble starch and peptone,the optimal temperature was 30 ℃,and the optimal pH value was 7.The light had no obvious effect to mycelium,while the dark culture conditions in 24 h were suitable for the spore production,and mycelial lethal temperature was 61 ℃ for 10 min.Conclusion： Fusarium oxysporum f.sp.ciceris isolate is reported as the pathogen of root-rot disesse in Saussurea lappa for the first time.

关 键 词：木香 根腐病 病原菌鉴定 生物学特性 

分 类 号：R282.2[医药卫生—中药学]