HPLC-ELSD法检测芪苈强心胶囊中3种成分的含量  

HPLC-ELSD Method Detected Three Components in Qiliqiangxin Capsule

作　　者：覃荣周 王琪林[2] 罗清扬 戴先芝 程敏 

机构地区：[1]阿坝师范学院藏羌医药研究所,四川阿坝623001 [2]成都体育学院运动医学与健康研究所,四川成都610041 [3]阿坝师范学院化学化工与生命科学系,四川阿坝623001 [4]阿坝师范学院附属医院中医科,四川阿坝623001

出　　处：《中药材》2017年第5期1134-1136,共3页Journal of Chinese Medicinal Materials

基　　金：四川省教育厅及省社科联基地2014年度课题(QXJ1301);阿坝师范学院2014专项基金课题(JY14-02)

摘　　要：目的:建立HPLC-ELSD法同时检测芪苈强心胶囊中槲皮素、山柰酚和异鼠李素的含量。方法:采用HPLC-ELSD法,Phenomenex C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.4%磷酸溶液(45∶55);流速:1.0m L/min;柱温:30℃;检测波长:360 nm。结果:槲皮素、山柰酚和异鼠李素的保留时间分别为5.3、9.3和12.2 min;测定方法的精密度、稳定性、重复性和加样回收率较好。5个批号的芪苈强心胶囊中槲皮素、山柰酚和异鼠李素的含量分别为0.53~0.63 mg/g、0.19~0.28 mg/g和0.15~0.21 mg/g。结论:该方法为芪苈强心胶囊的质量控制提供了参考。Objective： To study the HPLC-ELSD simultaneous detection of quercetin,kaempferol and isorhamnetin in Qiliqiangxin capsule.Methods： Using HPLC-ELSD method,Phenomenex C_（18） chromatographic column（250 mm × 4.6 mm,5 μm） was used for separation,the mobile phase was methanol-0.4% phosphoric acid solution（45 ∶ 55）,the flow rate was 1.0 m L/min,the column temperature was 30 ℃,the detection wavelength was 360 nm.The contents of quercetin,kaempferol and isorhamnetin were detected in five batches of Qiliqiangxin capsule.Results： The retention time of quercetin,kaempferol and isorhamnetin was 5.3,9.3 and 12.2 min,respectively.The precision,stability,repeatability and average recovery of determination method was good.The contents of quercetin,kaempferol and isorhamnetin in five batches Qiliqiangxin capsule were 0.53 ~ 0.63 mg/g,0.19 ~ 0.28 mg/g and 0.15 ~ 0.21 mg/g,respectively.Conclusion： This method can provide a reference in quality control of Qiliqiangxin capsule.

关 键 词：HPLC-ELSD 芪苈强心胶囊 槲皮素 山柰酚 异鼠李素 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]