锁阳籽油的超临界CO2萃取及其成分分析  

Optimization of Supercritical CO_2 Extraction and Composition Analysis of Cynomorium songaricum Seed Oil

作　　者：邓娟[1,2] 王旭 甘霖 王劼 孙菁 周玉碧[1] 

机构地区：[1]中国科学院藏药研究重点实验室/青海省青藏高原特色生物资源研究重点实验室,中国科学院西北高原生物研究所,青海西宁810008 [2]中国科学院大学,北京100049 [3]阿拉善盟科技局信息研究所,阿拉善750399

出　　处：《中药材》2017年第5期1150-1153,共4页Journal of Chinese Medicinal Materials

基　　金：青海省基础研究计划项目(2016-ZJ-765);阿拉善盟科技计划项目(A-4);青海省重点实验室发展建设专项(2017-ZJ-Y10)

摘　　要：目的:优化锁阳籽油的萃取条件并分析成分。方法:通过响应面设计优化超临界CO_2流体萃取锁阳籽油的试验条件,采用GC-MS方法对锁阳籽油进行成分分析。结果:优化的锁阳籽油超临界流体萃取条件为:萃取压力29 Pa、萃取温度37℃、CO_2流量20 m L/min、萃取时间2.5 h,在此条件下萃取率为4.71%。同时,对最优条件下所获萃取物进行了GC-MS分析,共鉴定出34种化合物。锁阳籽油中脂肪酸含量达97.69%,主要成分为反式油酸(35.589%)、十六烷酸(14.993%)、8,10-二甲氧基十八烷酸(11.633%)。结论:应用优化所得萃取条件萃取锁阳籽油,萃取率相对较高。锁阳籽油中以不饱和脂肪酸和甲氧基酸为主,二者含量共占提取物的66.61%。Objective： To optimize the process of supercritical CO_2 extraction and to analyze the compositions of Cynomorium songaricum seed oil.Methods： The process of supercritical CO_2 extraction of Cynomorium songaricum seed oil was optimized by response surface methodology,and the chemical compositions of the seed oil were analyzed by GC-MS method.Results： The optimal and viable condition of extraction were as follows,the extraction pressure was 29 MPa,the extraction temperature was 37 ℃,the CO_2 flowrate was20 m L/min,and the extraction time was 2.5 h.The yield of Cynomorium songaricum seed oil was 4.71%.In addition,34 compositions were identified by GC-MS by from Cynomorium songaricum seed oil.The content of fatty acid in Cynomorium songaricum seed oil was up to 97.69%.The main composition was（Z）-9-octadecenoic acid（35.589%）,hexadecanoic acid（14.993%） and 8,10-dimethoxyl octadecanoic acid（11.633%）.Conclusion： In the optimization condition of extraction can increase the yield of Cynomorium songaricum seed oil.Cynomorium songaricum seed oil have high content in unsaturated fatty acids and methoxy acid,two of them accounted for66.61% of the total extract.

关 键 词：锁阳籽油 超临界流体萃取 GC-MS 响应面优化 

分 类 号：R283.6[医药卫生—中药学]