黄芪配方颗粒对衰老大鼠认知功能的影响及机制初探  

Explore the Effect and Mechanism of Astragalus Particles in Aging Rats with Cognitive Impairment

作　　者：傅晓燕[1] 雷珊珊[2] 李雄英[1] 

机构地区：[1]杭州市第三人民医院,浙江杭州310009 [2]浙江中医药大学,浙江杭州310004

出　　处：《中药材》2017年第5期1184-1189,共6页Journal of Chinese Medicinal Materials

基　　金：浙江省中西医结合学会科研项目(2014LYZD001);杭州市卫生科技计划项目(2014A30)

摘　　要：目的:观察黄芪配方颗粒对自然衰老大鼠记忆认知作用的影响。方法:20月龄衰老大鼠随机分为自然衰老组、盐酸多奈哌齐(0.5 mg/kg)阳性对照组及黄芪配方颗粒高(0.450 g/kg)、中(0.275 g/kg)、低(0.138 g/kg)剂量组,连续给药干预12 w。给药10 w,采用Morris水迷宫实验检测空间记忆能力;给药12 w后处死大鼠,检测血清和脑组织SOD、MDA、CAT水平;HE染色观察海马区病理变化,免疫组化法观察脑组织Nrf2阳性表达;Western blot法检测脑组织Nrf2、HO-1蛋白表达水平。结果:黄芪配方颗粒高剂量组自主活动次数显著增加,逃避潜伏期、到达目的象限总路程及到达目的象限时间显著减少,血清及脑组织中SOD、CAT活性显著升高,MDA含量显著降低,脑组织HO-1、Nrf2蛋白表达显著升高(P<0.05或P<0.01),海马神经元变性明显改善,脑组织Nrf2阳性细胞表达显著增多。结论:黄芪配方颗粒可改善自然衰老大鼠模型的学习记忆功能,其作用机制可能与改善脑组织海马结构及Nrf2/ARE抗氧化信号通路密切相关。Objective： To study the effect and mechanism of Astragalus particles on improving memory and cognitive effects.Methods： Rats were randomly divided into control group,donepezil hydrochloride group（0.5 mg/kg）,high-dose Astragalus particles group（HQKL-H,0.450 g/kg）,moderate-dose Astragalus particles group（HQKL-M,0.275 g/kg） and low-dose Astragalus particles group（HQKL-L,0.138 g/kg）.Rats were given correspond drugs for continuous 12 w.After administrated for 10 w,the space memory capacity was detected by the test of Morris water maze.Rats were executed after administrated for 12 w,the autonomic activities were measured.The levels of SOD,MDA and CAT in serum and brain were detected by biochemical process and ELISA assay.The morphological changes in hippocampus tissue were detected by HE staining,and the positive expression of Nrf2 was measured by immunohistochemical method,the protein expression level of Nrf2 and HO-1 were measured by Western blot.Results： HQKL-H could significantly increase the autonomic activities,the level of SOD and CAT in serum and brain were significantly increased,the protein expression of HO-1 and Nrf2 were significantly increased（P 〈0.05 or P 〈0.01）,while the escape latency,total distance and time in the fourth quadrant,and the level of MDA were significantly decreased.Compared with aging model group,HQKL-H significantly increased the protein positive expression of Nrf2 in brain,and improving the nerve cell in hippocampus tissue.Conclusion： Astragalus particles can improve the learning capacity,the mechanism may be relevant to suppress the lesion in hippocampus and the anti-oxidative pathway of Nrf2/ARE.

关 键 词：黄芪配方颗粒 Nrf2/ARE信号通路 抗氧化 记忆功能 

分 类 号：R285.5[医药卫生—中药学]