鸡miR-125b的组织发育性变化及其功能预测分析  

Expressions of miR-125b in the tissues of Gushi chickens at developmental stages and prediction of its function

作　　者：闫峰宾 张蒙 孙桂荣 蒋瑞瑞 李转见 韩瑞丽 康相涛 李国喜 

机构地区：[1]河南农业大学牧医工程学院/河南省家禽种质资源创新工程研究中心

出　　处：《畜牧与兽医》2018年第2期43-51,共9页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31572356);教育部创新团队发展计划(IRT16R23);现代农业产业技术体系建设专项资金(CARS-41-K04)

摘　　要：为了解miR-125b的进化历史和潜在的生物学功能,下载不同物种miR-125b前体序列,运用Clustal W2软件进行多序列比对,构建系统进化树,q PCR方法检测固始鸡不同组织中miR-125b表达量的发育性变化,通过Targetscan 7.0与miRDB数据库对miR-125b靶基因进行预测、DAVID数据库对预测的靶基因进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析。结果显示：不同物种之间miR-125b具有很高的保守性;在胚胎期下丘脑中,随着胚胎发育,miR-125b的表达量逐渐增加,出壳后又呈现逐渐降低的趋势,到36周龄表达量又显著升高;在成年期消化器官（肌胃、腺胃、小肠、肝脏）中也具有较高的表达量;经预测,miR-125b靶基因有920个,富集于组织发育、生物调节、基因表达的转录后调节、小RNA的生物学加工等生物过程和细胞外基质受体相互作用、调节肌动蛋白细胞骨架、MAPK信号通路、胰岛素信号通路、不饱和脂肪酸的生物合成和NOD样受体信号等通路。结果表明：鸡miR-125b为广泛性时序表达miRNA,可能参与鸡胚胎期及出壳后生长发育相关的诸多生物学过程的调控。The present study was aimed to explore the evolutionary history and the potential biological functions of microRNA 125 b（miR-125 b）. Pre-miR-125 b sequences of various species were downloaded from the miRBase,multiple sequence alignments were performed using the Clustal W2 software,and the evolution tree of miR-125 b was constructed. To comprehensively understand the potential biological function of miR-125 b in chicken,the expression level of miR-125 b was detected in 15 different tissues of Gushi chickens at five developmental stages by qRT-PCR. The target genes for miR-125 b were predicted using Target Scan and miRDB,and were analyzed by GO（Gene Ontology）and KEGG pathway enrichment. Our results suggested that miR-125 b was highly conserved among different kinds of animals. In the embryonic stage of the thalamus,miR-125 b was gradually increased with the development of embryos,but decreased gradually after the shelling,and the expression level of the miR-125 b was significantly raised. In the adult digestive organs（muscle stomach,glandular stomach,small intestine,and liver） miR-125 b also exhibited a high expression level. Bioinformatics analysis showed that the predicted target genes of miR-125 b were significantly enriched in the biological processes of tissue development,biological regulation,post-transcriptional regulation of gene expression,biological processing of small RNAs,extracellular matrix receptor interaction,and regulation of actin cytoskeleton,and in the MAPK signaling pathway,insulin signaling pathway,unsaturated fatty acid biosynthesis and NOD-like receptor signaling pathway. In conclusion,chicken miR-125 b is ubiquitously expressed in all tissues examined and has temporal characteristic,and it may play an important role in the biological processes that are related to the development of many organs in hatched chicks.

关 键 词：鸡 miR-125b 进化 下丘脑 通路分析 

分 类 号：S831.2[农业科学—畜牧学]