丹参、泽泻对非酒精性脂肪肝大鼠脂代谢及纤溶系统的影响  

Effects of Salvia Miltiorrhiza and Rrhizoma Alismatis on Lipid Metabolism and Fibrinolytic System in Rats with Non-alcoholic Fatty Liver

作　　者：韩雪[1] 段思明 张兴芳 王茜[1] 刘宇[1] 郝蕾[1] 石铖[1] 张一昕[1] 

机构地区：[1]河北中医学院,石家庄050200

出　　处：《中国药师》2018年第2期211-214,共4页China Pharmacist

基　　金：河北省高等学校科学技术研究项目(编号:QN2015251);河北省中医药管理局科研计划项目(编号:2017025);国家级大学生创新创业训练项目(编号:201614432002)

摘　　要：目的:观察丹参、泽泻对非酒精性脂肪肝大鼠肝脏纤溶系统的影响。方法:采用喂饲高脂饲料复制非酒精性脂肪肝模型,将60只Wistar大鼠随机分为6组,正常组、模型组、丹参组,泽泻组,丹泽组和东宝肝泰对照组,正常喂养1周后,除正常组和模型组灌胃给予蒸馏水外,其余各给药组分别灌胃给予丹参水煎剂、泽泻水煎剂、丹参配伍泽泻水煎剂及东宝甘泰片治疗,8周后,检测血清总胆固醇(TC)、三酰甘油(TG)、游离脂肪酸(FFA)含量,免疫组化方法观察丹参、泽泻和丹泽水煎剂对纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂(PAI-1)的影响。结果:模型组大鼠血清TC、TG、FFA的含量以及PAI-1的表达明显升高(P<0.05或P<0.01),t-PA的表达明显降低(P<0.01)。各治疗组大鼠血清TC、TG、FFA的含量以及PAI-1的表达均降低(P<0.01),t-PA的表达升高(P<0.01)。结论:丹参、泽泻及丹参配伍泽泻水煎剂对非酒精性脂肪肝的治疗作用可能通过调整纤溶系统的失衡而实现,其机制可能与降低脂代谢有关。Objective： To preliminarily discuss the mechanism of Salvia miltiorrhiza and Rrhizoma alismatis in the prevention and treatment of lipid metabolism through regulating the imbalance of fibrinolytic system in rats with non-alcoholic fatty liver. Methods：The model of non-alcoholic fatty liver in rats was duplicated by high-fat diet feeding. Totally 60 adult Wistar rats were randomly divided into 6 groups（ n = 10 per group）,namely the control group,the model group,Salvia miltiorrhiza group,Rrhizoma alismatis group,Salvia miltiorrhiza and Rrhizoma alismatis group,and Dongbaogantai group. The control group and the model group were given distilled water,and the other group was given Salvia miltiorrhiza,Rrhizoma alismatis,Salvia miltiorrhiza combined with Rrhizoma alismatis and Dongbaogantai,respectively. Rhizoma Alismatis decoction at high dose and low dose as well as Dongbaogantai was respectively administrated in the designed groups,and the corresponding indices were detected 4 weeks later. Results： In the model control group,the contents of TC、TG、FFA in serum and the expression of PAI-1 were significantly elevated（ P 0. 05 or P 0. 01）,while the expression of t-PA was significantly reduced（ P 0. 01）. After treatment,the contents of TC、TG、FFA in serum and the expression of PAI-1 in every treatment groups were reduced（ P 0. 01）,while the expression of t-PA was elevated（ P 0. 01）. Conclusion： Increasing the expression of t-PA and reducing the expression of PAI-1 might be viewed as the mechanisms of Salvia miltiorrhiza and Rrhizoma alismatis in the prevention and treatment of non-alcoholic fatty liver disease.

关 键 词：非酒精性脂肪肝 丹参 泽泻 脂代谢 纤溶酶原激活物 纤溶酶原激活物抑制剂 

分 类 号：R285.5[医药卫生—中药学]