半胱胺修饰的FePt纳米颗粒对HeLa细胞的毒性  

Cytotoxicity of Cysteamine Modified FePt Nanoparticles in HeLa Cells

作　　者：张雷 孙英明 全红[1] 谢丛华[2] 

机构地区：[1]武汉大学物理科学与技术学院,湖北武汉430072 [2]武汉大学中南医院放化疗科,湖北武汉430071

出　　处：《武汉大学学报（理学版）》2017年第6期513-517,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金(81572967);医科达-武汉大学医学物理教学科研基金(250000200)资助项目

摘　　要：为探究半胱胺修饰的FePt纳米颗粒的抗肿瘤特性,本文采用化学还原法,制备出直径约3nm的FePt纳米颗粒,再用半胱胺(cysteamine,Cys)重新修饰其表面,将得到的FePt-Cys纳米颗粒分别与HELF(人胚肺成纤维细胞)和HeLa细胞(人宫颈癌细胞)孵育,用CCK-8法评估其细胞毒性,并通过流式细胞仪分析其对两种细胞周期分布的影响.结果发现,在一定浓度范围内,FePt-Cys纳米颗粒可以显著抑制HeLa细胞的增殖,但不影响HELF细胞的生长,其机制可能是通过细胞周期调节作用来产生细胞毒性.基于其对肿瘤细胞显著的杀伤作用和对正常细胞良好的生物相容性,FePt-Cys纳米颗粒将来可能作为一种有效的化疗药剂.In order to investigate the anti-tumor properties of cysteamine（Cys）modified FePt nanoparticles（NPs）,a chemical reduction method was implemented to synthesize the 3 nm NPs.With cysteamine remodified,the cytotoxicity of FePt-Cys NPs was studied by CCK-8 assay in HELF（human embryonic lung fibroblast cells）and HeLa cells（human cervical cancer cells）.In addition,to explore the detailed cytotoxic mechanism,cell-cycle redistribution analysis was conducted in both cell lines.The results indicate that FePt-Cys NPs could cause significant cytotoxicity in HeLa cells by cell-cycle regulation,but show nontoxicity to HELF cells below a proper concentration in the meantime.Given the remarkable inhibiting effect in tumor cells while a good biocompatibility to normal cells,FePt-Cys NPs could serve as an effective agent in future chemotherapy.

关 键 词：FePt-Cys纳米颗粒 HELF细胞 HELA细胞 细胞毒性 细胞周期调制 

分 类 号：Q279[生物学—细胞生物学]