点阵激光对豚鼠皮肤干细胞因子及其c-kit受体表达的影响  被引量:1

Effect of fractional laser on the expression of SCF and c-kit in skin stem cells of guinea pig

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作  者:谭明[1] 段波[1] 叶松[1] 吴丹[1] 周阎施婧[1] 

机构地区:[1]湖北省妇幼保健院医疗美容科,武汉430070

出  处:《中华医学美学美容杂志》2017年第6期415-418,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基  金:湖北省自然科学基金计划项目(2011CDC085)

摘  要:目的 探讨点阵激光不同能量对豚鼠皮肤干细胞因子(stem cell factor,SCF)及其c-kit受体表达的影响.方法 将20只豚鼠背部皮肤划分成A、B、C、D4个区域,分别采用不同参数处理:A区(对照区),不予激光照射;B区(低能量区),以20 mJ/cm2的能量照射;C区(中等能量区),以60 mJ/cm2的能量照射;D区(高能量区),以120 mJ/cm2的能量照射.激光处理后1周、4周2次分别切取不同治疗区皮肤,以免疫组织化学的方法检测SCF及c-kit受体的表达,并应用HPIAS-1000型全自动医学彩色图像分析系统进行定量分析.结果 激光处理后1周时,B、C、D组SCF的表达高于A组,差异有统计学意义(P<0.05);C、D组的c-kit受体表达高于A组,差异有统计学意义(P<0.05).4周时,C、D组SCF的表达高于A、B组,且D组高于C组,差异有统计学意义(P<0.05);D组c-kit受体的表达高于A、B二组,差异有统计学意义(P<0.05).结论 点阵激光能够促进SCF及c-kit受体的表达,且在一定范围内,能量越高促进作用越明显.Objective To investigate the effect of fractional laser on the expression of stem cell factor (SCF) and c-kit receptor in body surface of guinea pig.Methods 20 guinea pigs were adopted.The back skin of each guinea pig was divided into four zones:A zone (the skin was not treated),B zone (the skin was treated by fractional laser at a low fluence:20 mJ/cm2),C zone (the skin was treated at a medium fluence:60 mJ/cm2),and D zone (the skin was treated at a high fluence:120 mJ/cm2).The immunohistochemical method was used to detect the expression of SCF and c-kit in body surface of guinea pig.The results were quantified using an HPIAS-1000 system.Results One week later,the SCF expression level in B,C and D groups was increased obviously,which showed statistical difference compared with A group (P〈0.05);the c-kit expression level in C and D groups was increased obviously,compared with A group (P〈0.05).4 weeks later,the SCF expression level in C and D groups was higher than A and B groups;furthermore the level in D group was higher than C group (P〈0.05);the c-kit expression level in D group was higher than A and B groups (P〈0.05).Conclusions The fractional laser could generate the curative effect by increasing the expression of SCF and c-kit in body surface.

关 键 词:点阵激光 干细胞因子 C-KIT受体 豚鼠 

分 类 号:R622[医药卫生—整形外科]

 

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