2014-2015年昆明市杯状病毒分子流行特征研究  

Molecular epidemiological characteristics of Human Calicivirus in Kunming city, 2014-2015

作　　者：何李英 周永明[3] 古文鹏[3] 许万福 张顺先 艾琳[4] 卢艳[4] 田利光[4] 

机构地区：[1]暨南大学第一临床医学院,广州510630 [2]广州市妇女儿童医疗中心消化科,510623 [3]云南省疾病预防控制中心,昆明650022 [4]中国疾病预防控制中心寄生虫病预防控制所,上海200025

出　　处：《中华实验和临床病毒学杂志》2017年第6期525-529,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81473022）

摘　　要：目的 通过分析云南省昆明市＜5岁腹泻儿童杯状病毒（Human Calicivirus,HuCV）的分子流行特征,为HuCV相关腹泻的防治提供科学参考.方法 收集云南省昆明市4个哨点医院＜5岁腹泻儿童（n =850）和非腹泻儿童（n=170）的粪便样本,提取病毒RNA,采用逆转录聚合酶链反应（RT-PCR）检测杯状病毒,通过测序确定是否为阳性及亚型和基因型.结果HuCV的阳性率在腹泻儿童中高于非腹泻儿童（11.5％,98/850;4.7％,8/170,x2=7.083,P=0.008）,其中诺如病毒（Norovirus,NoVs） GⅡ阳性率在腹泻儿童中高于非腹泻儿童（11.1％,94/850;4.7％,8/170,x2=6.353,P=0.012）.而NoVs GⅠ（0.1％,1/850;0.0％,0/170,P=0.833）和扎如病毒（0.4％,3/850;0.0％,0/170,P=0.578）在腹泻人群和非腹泻人群中差异没有统计学意义.在腹泻人群和非腹泻人群中检出的102株NoVs GⅡ中,GⅡ.P4（10％,n=102）是最主要的基因型.NoVs GⅡ的阳性率在腹泻儿童中性别和年龄差异无统计学意义（x2=0.038,P=0.846;x2=0.620,P=0.733）,但存在明显的季节性差异（x2=9.867,P=0.020）,其流行高峰为秋季（15.6％）,以GⅡ.P4和GⅡ.P12株型尤其显著（x2=8.881,P=0.031;x2=7.917,P=0.039）.结论 昆明市腹泻儿童中NoVs GⅡ的流行程度较高,多个基因型均可引起感染,以GⅡ.P4为优势,同时出现了新的基因型GⅡ.P17.Objective To investigate the molecular epidemiological characteristics of Human Calicivirus （HuCV） infection among children less than 5 years in Kunming city,Yunnan province,it might be provide effective evident for prevention and control the diarrhea related with HuCV infection.Methods Four sentinel hospitals were recruited in the study from Kunming city,Yunnan province,850 diarrhea cases and 170 non-dianhea subject were recruited in this study from 2014 to 2015.RT-PCR was performed to screen HuCV infection,and gene sequencing was used to ensure positive infection subtypes and genotypes.Results The positive rate of HuCV was higher in children with diarrhea than in non-diarrhea children （11.5％,98/85;4.7％,8/170,x2 =7.083,P =0.008）,and the positive rate of Norovirus （NoV） GⅡwas higher in non-diarrhea children were （11.1％,94/85;4.7％,8/170,x2 =6.353,P =0.012）.There was no significant difference in the positive rate of NoV GⅠ （0.1％,1/850;0.0％,0/170,P =0.833） and Sappovirus （0.4％,3/850;0.0％,0/170,P =0.578） in diarrhea children and non-diarrhea children.GⅡ.P4 （10％,n =102） was the most important genotype of NoV GⅡ detected in diarrhea and non-diarrhea individuals.Despite no significant difference in Norovirus GⅡ infection between different age groups （x2 =0.038,P =0.846） and sex（x2 =0.620,P =0.733）,infection rate of NoV GⅡ varied with season （x2 =9.867,P =0.020）,having close relationship with diarrhea in autumn （15.6％）,primarily caused by GⅡ.4 and GⅡ.12 genotype （x2 =8.881,P =0.031;x2 =7.917,P =0.039）.Conclusions NoV GⅡ diarrhea had higher epidemic rate,which was caused by multiple genotypes,GⅡ.P4 was a dominant genotype,and was a major pathogenic agent of diarrhea in infants f in Kunming city.

关 键 词：杯状病毒 诺如病毒 腹泻 儿童 

分 类 号：R440[医药卫生—诊断学] R725.1[医药卫生—临床医学]