辣椒种质遗传多样性分析  

Assessment of Genetic Diversity of Capsicum Germplasms by SSR Markers

作　　者：李宁[1] 王飞[1] 尹延旭[1] 姚明华[1] 焦春海[2] 邹雄 赵荣秋[3] 高升华 

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430064 [2]湖北省农业科学院,武汉430064 [3]长江大学园艺园林学院,湖北荆州434023

出　　处：《辣椒杂志》2017年第4期1-8,13,共9页Journal of China Capsicum

基　　金：国家重点研发计划资助(2017YFD0101903;2016YFE0205500);国家自然科学基金项目资助(31401868);湖北省技术创新专项资助(2016ABA095;2016ABA087);现代农业产业技术体系建设专项资金资助(CARS-23-G28)

摘　　要：采用SSR标记方法,应用20对多态性SSR引物对311份国内外辣椒种质的DNA进行扩增,统计电泳检测结果,使用Powermaker 3.25软件计算等位基因数和变异范围,采用Neighbor-Joining法应用DARwin 6.0软件进行聚类分析,STRUCTURE 2.3软件进行群体遗传结构分析。结果表明,20对引物共扩增出263个等位基因,每个位点的变异范围为2~16个,平均每位点13.15个等位基因,每个位点PIC值变异范围为0.12~0.91,平均为0.823;基于Neighbor-Joining聚类方法将311份辣椒种质分为3组;进一步群体结构分析可将种质划分为3个群体,不同群体间的界限十分明显,且群体间的基因渗透较高。In this study, SSR markers were used to analyze the genetic diversity and genetic similarity of 311 domestic and foreign capsicum germplasms, the number of alleles and variation range estimated by Powermaker 3.25, the clustering analysis done by DARwin 6.0 with Neighbor-Joining, and the population genetic structure analyzed by the STRUCTURE 2.3 software. The results showed that 263 alleles were amplified by 20 pairs of polymorphic SSR primers, the number of alleles per locus ranged from 2 to 16 with an average of 13.15, and the PIC per locus changed from 0.12 to 0.91 and the average was 0.823. The 311 capsicum germplasms were classified into 3 groups with the Neighbor-Joining. The tested capsicum germplasms were also divided into 3 populations based on the population structure analysis. There was obvious introgression in the 3 groups.

关 键 词：辣椒 种质资源 遗传多样性 群体结构 SSR分析 

分 类 号：S641.3[农业科学—蔬菜学]