甘草断面颜色与其有效成分的相关性分析  

Correlation Analysis on Cross Section Color of Licorice and Its Active Ingredients

作　　者：米文娟[1] 林相龙 李阳[1] 陈慧荣 闫永红[1] 邹慧琴[1] 

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京宝德润生医药科技发展有限公司,北京100088

出　　处：《世界科学技术-中医药现代化》2017年第11期1829-1835,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金青年项目:电子鼻技术快速无损评鉴阳春砂"真伪优劣"的基础研究(81403054);主持人:邹慧琴;国家自然科学基金面上项目:基于形态特征;药效物质及药理作用变化规律采用电子鼻建立苦杏仁"走油"预警机制及质量实时监测系统(81573542);主持人:闫永红

摘　　要：目的:研究甘草的颜色值与有效成分含量之间的相关性,通过分析甘草断面颜色来推断内在成分含量情况。方法:采用色度计对甘草去皮打粉后的粉末进行颜色测量,同时对甘草中五种有效成分进行含量测定,包括高效液相色谱法测定甘草苷和甘草酸、比色法测定总皂苷、紫外分光光度法测定总黄酮、硫酸-苯酚比色法测定多糖。将断面颜色测量值与含量测定结果进行相关性分析。结果:甘草酸含量与L~*的相关系数为-0.578,P<0.001,与b~*的相关系数为0.596,P<0.001;甘草苷含量与L~*的相关系数为-0.503,P=0.002,与b~*的相关系数为0.890,P<0.001;总黄酮含量与L~*的相关系数为-0.729,P<0.001,与b~*的相关系数为0.724,P<0.001;多糖含量与L~*的相关系数为0.230,P=0.190,与b~*的相关系数为-0.390,P=0.023;总皂苷含量与L~*的相关系数为-0.411,P=0.016,与b~*的相关系数为0.738,P<0.001。结论:甘草断面颜色指标与甘草酸、甘草苷、总黄酮、总皂苷含量存在显著相关关系,而与多糖含量相关性不明显。证明甘草断面颜色与其内在成分密切相关,通过颜色的数字化测定可以客观地初步判断或预测甘草的中化学成分的含量,为中药材的质量优劣评价提供一种新思路、新方法。This study was aimed to establish an objective and convenient method to evaluate the quality of licorice through the study on correlation between the cross section color and contents of active ingredients of licorice. Therefore,colorimeter was introduced and applied to determinate cross section color of licorice. Meanwhile, contents of five active ingredients of licorice were also determined. HPLC was used to determine liquiritin and glycyrrhizic acid. Colorimetric method was used to determine total saponins. Ultraviolet spectrophotometry was used to determine total flavonoids.Sulphuric acid-phenol colorimetry was used to determine polysaccharides. Correlation between the cross section color and content determination result was analyzed. The results showed that the correlation coefficient of glycyrrhizic acid content and L-＊ was-0.578, P〈0.001, the correlation coefficient with b-＊ was 0.596, P〈0.001; the correlation coefficient of liquiritin content and L-＊ was-0.503, P = 0.002, the correlation coefficient with b-＊ was 0.890, P〈0.001;the correlation coefficient of total flavonoids content and L-＊ was-0.729, P〈0.001, the correlation coefficient with b-＊was 0.724, P〈0.001; the correlation coefficient of polysaccharides content and L-＊ was 0.230, P = 0.190, the correlation coefficient with b-＊ was-0.390, P = 0.023; the correlation coefficient of total saponins content and L-＊ was-0.411, P =0.016, the correlation coefficient with b-＊ was 0.738, P〈0.001. It was concluded that the cross section color index of licorice has significant correlation with contents of glycyrrhizic acid, liquiritin, total flavonoids and total saponins. There was no significant correlation with content of polysaccharides. It illustrated the close correlation between cross section color of licorice and its active ingredients. Through the digitalized determination on color, contents of chemical composition in licorice can be initially determined or predicted objectively. It provided a new idea and method for the q

关 键 词：色度计 甘草 断面颜色 有效成分 相关性 

分 类 号：R282[医药卫生—中药学]