小剂量阿司匹林对去势骨质疏松大鼠钛种植体骨结合的影响  

Effect of low dose aspirin on osseointegration around titanium implants in osteoporotic rats

作　　者：杨琪 李风兰 

机构地区：[1]山西医科大学附属人民医院口腔修复科,太原030012

出　　处：《中华口腔医学杂志》2018年第2期92-96,共5页Chinese Journal of Stomatology

基　　金：山西省重点研发计划（201703D321027-2）

摘　　要：目的探讨小剂量阿司匹林对去势骨质疏松大鼠钛种植体骨结合的影响，为阿司匹林在口腔种植临床的应用提供依据。方法选3~4个月龄雌性SD大鼠60只，分为卵巢切除组48只，假手术组12只。卵巢切除组切除大鼠双侧卵巢，假手术组行假手术。术后12周验证造模成功后，将卵巢切除组分为4个亚组（每组12只）：骨质疏松组和3个阿司匹林干预组（A1、A2、A3组）。所有大鼠胫骨干骺端植入钛种植体，植入后3 d A1、A2、A3组分别按阿司匹林2.06、4.11、8.21 mg·kg^-1·d^-1灌胃。骨质疏松组和假手术组等量0.9%氯化钠注射液灌胃，1次/d。植入后4、12周，每组处死6只大鼠，测量种植体骨结合率（implant bone connect rate，IBCR）、结合骨板宽度（combinded bone lamella width，CBLW）及骨小梁平均宽度（trabercular width，TW）。结果植入后4周，A1组TW和CBLW[（39.60±2.77）和（27.56±4.14）μm]，A2和A3组IBCR、TW和CBLW[A2：（47.21±4.19）%、（48.74±3.20）和（35.91±3.79）μm；A3：（47.35±6.07）%、（50.27±5.25）和（40.66±2.11）μm]均显著大于骨质疏松组相应指标[（33.89±7.17）%、（32.20±6.10）和（19.77±6.80）μm]（P〈0.05）；A3组CBLW与假手术组[（46.11±5.87）μm]差异无统计学意义（P〉0.05）。植入后12周，A1组IBCR和CBLW[（85.86±3.64）%、（53.12±8.68）μm]，A2和A3组IBCR、TW和CBLW[A2：（85.64±3.97）%、（69.42±6.78）和（54.19±3.12）μm；A3：（86.22±3.48）%、（75.43±3.50）和（55.79±5.60）μm]均显著大于骨质疏松组相应指标[（77.20±7.14）%、（55.10±2.26）和（41.77±3.13）μm]（P〈0.05）；A1、A2、A3组IBCR与假手术组[（90.09±2.21）%]差异无统计学意义（P〉0.05）。结论小剂量阿司匹林能提高去势大鼠种植体骨结合率、结合骨板平均宽度及种植体周围骨小梁的平均宽度。Objective To investigate the effect of aspirin on osseointegration around titanium implants in ostoeporotic rats and to provide evidence for future researches and clinical application.Methods A total of 60 female SD rats, aged 3-4 months, were divided into ovariectomy group （Ovx group, n=48） and sham-ovariectomy group （Sham group, n=12）. The rats in Ovx group received ovariectomy and those in Sham group underwent sham-ovariectomy. Twelve weeks later, six rats in each group were randomly selected to confirm the osteoporosis models. The Ovx group was divided into 4 subgroups with 12 rats in each group, namely the osteoporosis group （OP group）, and Aspirin groups （A1, A2, A3 group）. Pure screw titanium implants were placed in the right tibia near metaphysis of all rats. Three days after implant surgery, aspirin groups were intragastrically administered aspirin at a dose of 2.06, 4.11, 8.21 mg·kg^-1·d^-1 （A1, A2, A3）, and OP group and Sham group were fed the same amount of normal saline. Four and 12 weeks following implantations surgery, half of the rats in each group were randomly chosen and sacrificed. Implant bone contact rate （IBCR）, combined bone lamella width （CBLW） and trabercular width （TW） were observed and calculated using histomorphometric measurement.Results Four weeks after implantations surgery, the TW and CBLW of rats in A1 group [（39.60±2.77） and （27.56±4.14） μm] and the IBCR, TW and CBLW of rats in A2 group and A3 group [A2： （47.21±4.19）%, （48.74±3.20） and （35.91±3.79） μm; A3： （47.35±6.07）%, （50.27±5.25） and （40.66±2.11） μm] were much higher than those in OP group [（33.89±7.17）%, （32.20±6.10） and （19.77±6.80） μm]（P〈0.05）. In term of CBLW, there were no difference between A3 group and Sham group [（46.11±5.87） μm]（P〉0.05）. Twelve weeks after implantations surgery, the IBCR and CBLW of rats in A1 group [ （85.86±3.64） %, （53.12±8.68） μm], and the IBCR, TW and CBLW of rats in A2 gr

关 键 词：阿司匹林 牙种植体 骨质疏松 骨结合 

分 类 号：R580[医药卫生—内分泌] R783.6[医药卫生—内科学]