林蛙养殖场地土壤菌群结构及潜在病原菌分析  

Analysis of Soil Microbial Communities and Potential Pathogens in Rana dybowskii Farm

作　　者：别佳 佟庆[1,2] 胡宗福 崔立勇 刘笑凝 王洪斌 

机构地区：[1]东北农业大学动物医学学院,实验动物与比较医学重点实验室,哈尔滨150030 [2]黑龙江省合江林业科学研究所,佳木斯154002

出　　处：《中国畜牧兽医》2018年第2期528-535,共8页China Animal Husbandry & Veterinary Medicine

基　　金：林蛙集约化养殖防疫技术研究(sgzjY2010025)

摘　　要：试验旨在了解林蛙养殖场地土壤菌群组成和结构特征、喷洒益生菌后的土壤菌群变化和潜在致病菌的种类与数量。试验设置3个组:养殖场地组、益生菌喷洒组和半人工养殖组,用高通量测序技术进行检测。3个试验组共获得191 588条有效序列,1 369个操作分类单元(operational taxonomic units,OTU),经分类学鉴定分属于438个属,29个门。3组样本菌群数量较大,多样性水平也相对较高。优势菌门为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、蓝细菌门(Cyanobacteria)、芽单胞菌门(Gemmatimonadetes)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、厚壁菌门(Firmicutes);优势菌属为黄杆菌属(Flavobacterium)、热单胞菌属(Thermomonas)、Sphingopyxis、紫杆菌属(Porphyrobacter)、蓝细菌纲中未定种类(norank-c--Cyanobacteria)。各分组之间菌群多样性差异不显著,各组菌群结构相似度高。土壤中潜在的红腿病致病菌在属的水平有气单胞菌属(Aeromonas)、柠檬酸杆菌属(Citrobacter)、金黄杆菌属(Chryseobacterium)、假单胞菌属(Pseudomonas)。喷洒益生菌后土壤菌群变化不明显。本研究为全面了解东北林蛙养殖场地土壤微生物组成及变化、发掘有益微生物种类和东北林蛙疾病的预防和治疗提供理论依据。The aim of this study was to understand the composition and structural characteristics of soil microbial of Rana dybowskii,changes in microbes after spraying probiotics and the number of potential pathogens.Three experimental groups were set up：Breeding farm group,probiotics spraying group and semi-artificial breeding group.And high-throughput sequencing was used for detection.A total of 191 588 valid sequences and 1 369 OTUs were obtained from three experimental groups,which belonged to 438 genera and 29 phyla by taxonomy.The number of bacteria in three groups was relatively large,and the diversity level was relatively high.The dominant bacteria on phylum level were Proteobacteria,Bacteroidetes,Cyanobacteria,Gemmatimonadetes,Chloroflexi,Actinobacteria,Acidobacteria and Firmicutes;The dominant bacteria on genus level were Flavobacterium,Thermomonas,Sphingopyxis,Porphyrobacterand norank-c--Cyanobacteria.The diversity of microbial community among the groups did not reach the significant level.The similarity of the microbial communities was high.The potential red-leg syndrome pathogenic bacteria in the soil were Aeromonas,Citrobacter,Chryseobacteriumand Pseudomonas.The chan-ges of soil microbes after spraying probiotics were not obvious.This study provided the basis theory for comprehensively understanding the composition and variation of soil microbes of the Rana dybowskii farm,exploring the types of beneficial microorganisms and the prevention and treatment of Rana dybowskii.

关 键 词：东北林蛙 土壤 高通量 微生物 

分 类 号：S154.381[农业科学—土壤学]