基于HPLC-MS^n的甘草成分快速鉴定及质谱裂解途径研究  

Study on identification of compounds and their fragmentation pathways in licorice by HPLC-MS^n

作　　者：马海娟[1] 高简 张亚丽[2] 张潇[2] 苗祥贞 谭鹏[2] 

机构地区：[1]北京市第六医院,北京100007 [2]北京中医药大学,北京102488

出　　处：《中华中医药杂志》2018年第3期1120-1123,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81102807)~~

摘　　要：目的:采用高效液相色谱-质谱(HPLC-MS^n)法,对甘草提取物中的成分进行分析。方法:采用C_(18)色谱柱,乙腈-0.2%乙酸水溶液梯度洗脱,线性离子阱-串联静电轨道阱(LTQ-Orbit rap)高分辨质谱,负离子检测模式进行扫描,解析多级质谱数据,进行成分定性分析。结果:甘草提取物中共检测出31种成分,其中23种黄酮类化合物,8种三萜皂苷类成分,并首次分析了其中两种成分的裂解途径。结论:建立的HPLC-MS^n分析甘草提取物成分的方法快速简便,可用于复杂成分分析,为甘草的质量控制提供了参考依据。Objective： To establish an HPLC-MS^n method for identifying chemical constituents in extract of licorice. Methods： Chromatographic separation was achieved on a C18 column with the mobile phase of acetonitrile-water（0.2% acetic acid） and step gradient elution. A LTQ-Orbit rap mass spectrometers quantification was equipped with electrospray ionization source was used as detetor and operated in the negative ion mode. Results： Thirty-one compounds were identified from licorice, including 8 kinds of triterpenoid saponins and 23 kinds of flavanone.To analyze mass spectrometry fragments of Liquiritin and licorice-saponineunder the positive mode. Conclusion： The method is rapid and simple in the qualitative analysis of licorice using LC-LTQ-Orbit rap MS/MS. This study could be helpful for the quality control and further phytochemical studies of licorice.

关 键 词：甘草 液质联用 黄酮 三萜皂苷 

分 类 号：O657.63[理学—分析化学] R284[理学—化学]