Ⅱ型登革病毒前膜抗体对该病毒在THP-1细胞中复制能力的影响  

Effect of presynaptic membrane antibody on replication capacity of dengue virus Ⅱ in THP-1 cells

作　　者：姜黎明[1] 杨佳佳[1] 罗佳[1] 叶超[1] 文送娇 孙强明[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,云南昆明650118

出　　处：《中国生物制品学杂志》2018年第2期125-128,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金面上项目(81171946);国家"十二五""重大新药创新"科技重大专项(2012ZX09104-302);云南省科技厅社会发展科技计划项目(2011CA016);云南自然科学基金项目(2012FB188;2016FA029);医学生物学研究所研究生创新基金(2015-018-004)

摘　　要：目的探讨Ⅱ型登革病毒(dengue virusⅡ,DENVⅡ)前膜(presynaptic membrane,prM)抗体对DENVⅡ在THP-1细胞中复制能力的影响。方法采用不同稀释度(1/4~1/16 384)的DENVⅡanti-pr M单克隆抗体分别与不同MOI的DENVⅡ(MOI分别为3、0.75及0.19)复合感染THP-1细胞。建立DENVⅡ荧光定量PCR检测标准曲线,检测THP-1细胞培养上清液的病毒拷贝数。结果 DENVⅡ按MOI=3感染THP-1细胞,当prM稀释度为1/16和1/16 384时诱发THP-1细胞产生病毒的载量较高,prM稀释度为1/64和1/256时抑制病毒生长;DENVⅡ按MOI=0.75感染THP-1细胞,当prM稀释度1/16时可诱发更高浓度病毒载量;DENVⅡ按MOI=0.19感染THP-1细胞时,不会诱导产生高浓度的病毒载量。结论中浓度pr M抗体可抑制DENVⅡ在THP-1细胞中的复制能力,而高及低浓度prM抗体可促进DENVⅡ在THP-1细胞中的复制能力。Objective To investigate the effect of presynaptic membrane （prM） antibody on replication capacity of dengue virus Ⅱ （DENV Ⅱ ） in THP-1 cells. Methods THP-1 cells were co-infected with anti-prM monoclonal antibody of DENV- Ⅱ, at various dilutions （1/4 - 1/16 384） and DENV Ⅱ at various MOIs （3, 0. 75 and 0. 19）. The standard curve of real-time fluorescent quantitative PCR for DENV Ⅱ was plotted and used for determination of virus copy number in culture supernatant of THP-1 cells. Results High virus load was induced in THP-1 cells infected with DENV Ⅱ at a MOI of 3 and prM dilutions of 1 / 16 and 1 / 16 384, while the virus growth was inhibited when the dilutions of prM were 1 / 64 and 1 / 256. Higher virus load was induced in the cells infected with DENV Ⅱ at a MOI of 0. 75 and a prM dilution of 1 / 16. However, the DENV II at a MOI of 0. 19 induced no high virus load. Conclusion The prM antibody at a moderate concentration inhibited the replication capacity of DENV Ⅱ in THP-1 cells, while those at high and low concentrations nromoted the renlication canacitv.

关 键 词：Ⅱ型登革病毒 前膜 抗体 THP-1细胞 复制能力 

分 类 号：R373.33[医药卫生—病原生物学]