黄芪多糖在变应性哮喘大鼠模型中对树突状细胞的免疫干预作用  

Immune intervention of Astragalus polysacchaside to dendritic cells in rat model of allergic asthma

作　　者：刘璟 游进 

机构地区：[1]重庆市三峡医药高等专科学校,重庆404000 [2]重庆市三峡中心医院,重庆404000

出　　处：《中国生物制品学杂志》2018年第2期140-144,共5页Chinese Journal of Biologicals

基　　金：重庆市万州区科委自然科学类课题(201403052)

摘　　要：目的探讨黄芪多糖(Astragalus polysacchaside,APS)对变应性哮喘(allergic asthma,AS)大鼠模型树突状细胞(dendritic cells,DCs)的免疫干预作用。方法选用卵清蛋白(ovalbumin,OVA)对30只大鼠进行连续雾化致敏,建立AS模型,其中10只为模型组,20只经腹腔注射APS,给药量分为100和200μg/kg(各10只),同时以10只未建模的大鼠为对照组。给药4 h后观察大鼠的症状,并按预设评估标准评价治疗效果;给药24 h后检测各组大鼠肺功能,并处死大鼠,取肺黏膜相关淋巴组织,用免疫组织化学技术检测CD80、CD86、IL-4、IL-10、IL-12的表达情况。结果根据预设的评估情况,100μg/kg APS干预组9只显效,1只缓解,200μg/kg APS干预组10只显效。与对照组比较,模型组的第1秒肺活量(forced expiratory volume in one second,FEV1)/用力肺活量(forced vital capacity,FVC)比值、最大呼气流速(peak expiratory flow rate,PEF)及IL-10、IL-12表达水平均明显下降(P<0.05),CD80、CD86、IL-4的表达水平均显著升高(P<0.05);与模型组比较,100及200μg/kg APS干预组FEV1/FVC比值、PEF及IL-10、IL-12表达水平显著升高(P<0.05),CD86、CD80及IL-4的表达水平明显降低(P<0.05);100与200μg/kg APS干预组间各指标差异均无统计学意义(P>0.05)。结论 APS在AS大鼠模型中可通过调整DCs的表型表达缓解AS病情。Objective To investigate the immune intervention of Astragalus polysacchaside （APS） to the dendritic cells （DCs） in rat model of allergic asthma （AS）. Methods Thirty rats were sensitized with ovalbumin by continuous nebulization to establish the model of AS. Of the model rats, 20 were injected i. p. with APS at dosages of 100 and 200 lag/kg, 10 for each, while the other 10 as model control were untreated. Another 10 healthy rats were served as normal control. The symptoms of rats were observed 4 h after injection, and the curative effect was evaluated according to the presupposed standard. The lung functions of rats in various groups were determined 24 h after injection, while the rats were killed, of which the pulmonary mucosa associated lymphoid tissues were collected and determined for expressions of CD80, CD86, IL-4, IL-10 and IL-12 by immunohistochemical technique. Results According to the presupposed standard, remarkable effectiveness was observed in 9 rats in 100 lag/kg APS intervention group, while remission in one rat. However, remarkable effectiveness was observed in all the 10 rats in 200 μg/kg APS intervention group. Compared with those in normal control group, the forced expiratory volume in one second （FEV1）/forced vital capacity （FVC） ratio, peak expiratory flow rate （PEF） as well as expression levels of IL-10 and IL-12 in model control group decreased significantly （P 〈 0. 05）, while the expression levels of CDS0, CD86 and IL-4 increased significantly （P 〈 0. 05）. However, compared with those in model control group, the FEV 1/FVC ratio, PEF and as well as expression levels of IL- l0 and IL-12 in 100 and 200 μg/kg APS intervention groups increased significantly （P 〈 0.05）, while the expression levels of CD80 and IL-4 decreased significantly （P 〈 0. 05）, which showed no significant difference in two intervention groups （P 〉 0. 05）. Conclusion APS may relieve AS in rat model by adjusting the phenotype of DCs.

关 键 词：黄芪多糖 变应性哮喘 大鼠 树突状细胞 免疫干预 

分 类 号：R392.8[医药卫生—免疫学]