亲和吸附材料特异性清除肠源性内毒素有效性和安全性探讨  

Efficacy and safety of the removal of intestinal endotoxin in the blood by affinity adsorption materials

作　　者：向勇平 崔辉[2,3] 刘丹[2,3] 于杰 周立冬[4] 杨建明 刘爱兵 XIANG Yongping;CUI hui;LIU dan;YU jie;ZHOU Lidong;YANG Jianming;LIU Aibing(Postgraduate Training Base, the General Medical Experimental Center, Hospital of Chinese People＇s Armed Police Forces of Jinzhou Medical University, Beijing 100039, China;General Hospital of Chinese People＇ s Armed Police Forces, Beijing 100039;Beijing Key Laboratory of Disaster Rescue Medicine,Beijing 100039;Tianjin Yangquan Medical Instrument Limited Company, Tianjin 300350;Tianjin Port Hospital, Tianjin 300350)

机构地区：[1]锦州医科大学武警总医院研究培养基地,北京100039 [2]武警总医院医学实验中心,北京100039 [3]灾害救援医学北京市重点实验室,北京100039 [4]天津市阳权医疗器械有限公司,天津300350 [5]天津市港口医院,天津300350

出　　处：《中国实验动物学报》2018年第1期52-56,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家高技术研究发展计划(No.2015AA033904)~~

摘　　要：目的探究本课题组研发的亲和吸附材料特异清除肠源性内毒素的有效性及安全性。方法运用阑尾结扎穿孔的方法建立犬肠源性内毒素血症模型,进行灌流治疗。实验过程中选取多个时间点,监测生命体征,采血检测内毒素含量及血液生理生化指标。结果灌流吸附1、2 h后内毒素含量为(0.49±0.22)、(0.034±0.00)Eu/mL,与灌流开始(7.25±1.18)Eu/mL相比,差异有显著性(P<0.05)。灌流2 h后平均清除率R=99.52%。灌注治疗后白细胞、红细胞、血红蛋白、血小板、总蛋白、白蛋白、球蛋白、谷丙转氨酶、谷草转氨酶、尿素、肌酐指标均有所下降,与灌流开始相比差异有显著性(P<0.05),各值均在正常值范围。灌流过程中犬的生命体征平稳。结论本课题研发的亲和吸附材料能高效清除实验犬血液中内毒素并有良好的血液相容性。Objective To investigate the efficacy and safety of the affinity adsorption material for removal of intestinal endotoxin in the blood. Metheds To establish a canine model of intestine-derived endotoxemia by ligation and perforation of the appendix. Hemoperfusion was performed to treat the endotoxemia in model dogs. In the experiment,we monitored vital signs and detect the content of endotoxin and blood physiological and biochemical indexes at different time points. Results The endotoxin content was（ 0. 49 ± 0. 22）,（ 0. 034 ± 0. 00） Eu/m L after hemoperfusion for 1 and 2 hours,compared with that before the beginning of perfusion（ 7. 25 ± 1. 18） Eu/m L,showing a significant difference（ P〈0. 05）. After treatment for 2 hours,the average clearance rate was 99. 52%. White blood cells,red blood cells,hemoglobin,platelets,total protein,albumin,globulin,alanine aminotransferase,aspartate aminotransferase,urea and creatinine index were significantly decreased after perfusion（ P〈0. 05）. The index values were in a normal range and vital signs were stable during the perfusion. Conclusions The affinity adsorption material developed by our team can effectively remove endotoxin in the blood of experimental dogs and has good blood compatibility.

关 键 词：内毒素 肠源性内毒素血症 血液灌流 亲和吸附材料 犬 

分 类 号：Q95-33[生物学—动物学]