金思维对东莨菪碱致记忆障碍小鼠脑胆碱能系统的影响  

Effect of herbal formula GAPT on cholinergic system in mice with scopolamine-induced memory impairment

作　　者：董云芳[1] 冯慧利[1] 陈芳[1] 盛宁[1] 任映[1] 杨金铎[1] 时晶[2] 田金洲[2] 王蓬文[1,2] DONG Yunfang;FENG Huili;CHEN Fang;SHENG Ning;REN Ying;YANG Jingduo;SHI Jing;TIAN Jinzhou;WANG Pengwen(Key Laboratory of Chinese Internal Medicine, Beijing University of Chinese Medicine, Ministry of Education, Beijing 100700, China;Institute of Neurodegenerative Diseases, Beijing University of Chinese Medicine, Beijing 100700)

机构地区：[1]北京中医药大学东直门医院中医内科学教育部和北京市重点实验室,北京100700 [2]北京中医药大学神经变性病研究所,北京100700

出　　处：《中国实验动物学报》2018年第1期107-113,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京中医药大学在读研究生项目(No.2016-JYB-XS142);北京市教育委员会科学研究与研究生培养共建项目(No.2016;2017);国家自然科学基金面上资助项目(No.81573927)~~

摘　　要：目的通过建立东莨菪碱记忆障碍模型,采用中药复方金思维进行干预,观察金思维对东莨菪碱致记忆障碍模型小鼠行为学和胆碱能系统的影响,探讨该药的神经保护作用机制。方法将ICR小鼠随机分为正常对照组;模型组,溶媒0.5%CMC;阳性对照组,多奈哌齐,0.92 mg/(kg·d);金思维大、中、小剂量组,20、10、5 mg/(kg·d)。每组18只,按0.1 mL/10 g小鼠体重连续灌胃给药30 d。末次给药后造模,对照组腹腔注射生理盐水,其余各组腹腔注射东莨菪碱3 mg/(kg·d),溶于0.9%生理盐水,按0.1 mL/10 g小鼠体重注射,进行Morris水迷宫实验。实验结束后取皮层和海马组织,分别测定皮层和海马中乙酰胆碱(Ach)含量、乙酰胆碱酯酶(Ach E)及胆碱乙酰转移酶(Ch AT)活性。结果金思维可使模型小鼠游泳距离和游泳时间缩短及目标象限停留时间增长;金思维可使模型组小鼠脑内Ach含量升高、Ach E活性下降和Ch AT活性升高。结论金思维可以改善东莨菪碱导致的记忆障碍模型小鼠学习记忆能力,其机制可能与胆碱能能系统有关。Objective To investigate the effect of a Chinese medicine formula GAPT,a combination containing Chinese herbs ginseng,Acorus tatarinowii,Polygala and tuber curcuma,on the behavior and cholinergic system in mice with scopolamine-induced memory impairment,and to explore the neuroprotective mechanism of GAPT. Methods ICR mice were randomly divided into normal control group（ solution of 0. 5% carboxy methyl cellulose,CMC）,model group（ 0. 5% CMC）,positive control group（ donepezil,0. 92 mg/（ kg·d））,GAPT high,medium and low dose groups（ 20 mg/（ kg·d）,10 mg/（ kg·d）,5 mg/（ kg·d））,18 mice in each group,were given intragastric gavage once a day for 30 days. After the last administration,the control group and model group received intraperitoneal injection of normal saline,and the other groups intraperitoneal injection of scopolamine（ 3 mg/（ kg·d））,dissolved in 0. 9% normal saline. Morris water maze test was performed to assess the learning and memmory ability. Then the mice were killed and the Ach content and Ach E and Ch AT activity in the cortex and hippocampus were detected. Results GAPT increased the swimming distance,swimming time and the residence time in target quadrant of the model mice,increased the content of Ach and the activity of Ch AT,and decreased the activity of Ach E in the brain of the model group. Conclusions GAPT can improve the learning and memory ability of mice induced by scopolamine,and its mechanism may be related to cholinergic system.

关 键 词：金思维 阿尔茨海默病 乙酰胆碱 乙酰胆碱酯酶 胆碱乙酰转移酶 小鼠 

分 类 号：Q95-33[生物学—动物学]