石斛不同极性成分的提取及其对肺癌细胞的抑制作用  

Extraction of components with different polarities from Dendrobium and their inhibitory action on lung cancer cells

作　　者：李莹[1,2] 刘静 张雪琴[1,2] 何涛 淳泽[1] 胡亚东[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049

出　　处：《应用与环境生物学报》2018年第1期13-19,共7页Chinese Journal of Applied and Environmental Biology

基　　金：四川省“十三五”中药材育种攻关项目(2016NYZ0036-1);四川省应用基础研究项目(2015JY0265);四川省科技支撑计划项目(2014FZ0086、2016JZ0015);四川省科技基础条件平台项目资助~~

摘　　要：为探究石斛（Dendrobium）的不同极性成分对人肺癌细胞抑制作用的差异,寻找到石斛抗癌活性成分高效提取的方法,采用极性不同的溶剂和方法对金钗石斛（D.nobile）、迭鞘石斛（D.denneanum）的药用成分分别进行提取以及层析柱纯化,以CCK-8实验检测不同极性提取物对人肺癌细胞和正常细胞的抑制作用,同时采用傅里叶变换红外光谱仪分别对石斛的乙醚和乙醇提取物成分进行检测和比较.结果显示,石斛的不同极性成分抑癌作用差异显著,石斛的抑癌成分极性较小,易溶于乙醚.经石油醚预洗后的石斛乙醚提取物对肺癌细胞的抑制作用较强,100μg/mL的金钗石斛与迭鞘石斛提取物对肺癌A549细胞的24 h抑制率分别为45%和40%.而传统中药成分提取中常用的水和乙醇对石斛抗癌成分的提取效率较低.红外光谱分析亦显示石斛的乙醚提取物与乙醇提取物化学成分上存在较大差别,因此对于石斛抗癌成分的提取应采用极性较小的乙醚等溶剂.此外,发现金钗石斛的特定极性组分C具有较强的抑制肺癌细胞作用,其100μg/mL的浓度对肺癌A549细胞的24 h抑制率达到97%,可作为石斛抗癌新药筛选的来源.因此,金钗石斛和迭鞘石斛的抑癌成分具有特定的极性,结果可为石斛抗癌成分及抗癌作用的进一步研究和利用提供参考.The present study aimed to（1） explore the difference in the inhibitory action of components with different polarities extracted from Dendrobium on human lung cancer cells and（2） identify the efficient method to extract the anticancer component from Dendrobium. The medicinal components were extracted from D. nobile and D. denneanum using different polar solvents and by different extraction methods. The components were purified by chromatography. The inhibitory action of different extracts on human lung cancer cells A549 and human embryo lung cells MRC-5 was determined by CCK-8 assay. Furthermore, Fourier-transform infrared spectroscopy（FTIR） was used to detect the difference between the ether and ethanol extracts of Dendrobium. The results showed differences in the anticancer activity among the components with different polarities extracted from Dendrobium. The extraction of anticancer components from Dendrobium using water and ethanol as solvents was not efficient. Further, the components extracted using ether after the petroleum ether pre-washing exhibited the highest inhibitory action on A549 cells. The inhibition rate of 100 μg/m L ether extract from D. nobile and D. denneanumon lung cancer A549 cells was 45% and 40%, respectively, after 24 h. The FTIR spectrum analysis also indicated significant difference in the components among ether and ethanol extracts. Furthermore, most of the anticancer components were less polar. Therefore, the components can be extracted using weak polar solvents, such as ether. Besides, the study found that component C with specific polarity isolated from D. nobile had strong anticancer activity, and the inhibition rate of 100 μg/mL component C on lung cancer A549 cells was about 97% after 24 h. Therefore, it might be used as a source to develop anticancer drugs. These results suggest that the anticancer components of D. nobile and D. denneanum possess specific polarity, which can be exploited in further studies and develop anticancer drugs.

关 键 词：金钗石斛 迭鞘石斛 不同极性提取物 抑癌作用 乙醚提取物 肺癌细胞 

分 类 号：Q949.718[生物学—植物学] R962[医药卫生—药理学]