高压氧对急性一氧化碳中毒大鼠少突胶质细胞前体细胞ADAM10 mRNA表达的影响  

Hyperbaric oxygen on the expression of ADAM10 of rat oligodendrocyte precursors in vitro after acute carbon monoxide poisoning

作　　者：郭大志[1] 冯园[1] 胡慧军[1] 李娜[1] 潘树义[1] 

机构地区：[1]海军总医院高压氧科,北京100048

出　　处：《中国医药导报》2018年第5期9-12,共4页China Medical Herald

基　　金：北京市自然科学基金资助项目(7153175);国家自然科学基金资助项目(81401063)

摘　　要：目的探讨高压氧对急性一氧化碳中毒大鼠少突胶质细胞前体细胞ADAM10 mRNA表达的影响。方法体外纯化培养新生大鼠皮层少突胶质细胞前体细胞(OPCs),按3种处理方式分为对照组、急性一氧化碳中毒组(ACOP组)、高压氧组(HBO组)。对照组不进行任何处理;ACOP组将细胞放在密闭容器内,根据体积比例注入1%一氧化碳;HBO组将一氧化碳处理的OPCs放入动物实验舱中,以98%O_2、2%CO_2洗舱10 min,然后15 min内加压至0.35 MPa并停留2h,20 min内减至常压,处理期间舱温控制在37℃,余处理同ACOP组。以HBO处理后即刻为0h,三组均在6、24h和48h时间点取材;采用RT-PCR方法观察不同时间点OPCs细胞ADAM10 mRNA的表达。结果在6、24h和48h三个时间点,大鼠少突胶质细胞前体细胞ADAM10 mRNA在对照组无明显变化,ACOP组逐渐减少,HBO组逐渐增多,差异有统计学意义(F=8.491,P<0.01);与对照组在6、24h和48h三个时间点大鼠少突胶质细胞前体细胞ADAM10 mRNA水平比较,ACOP组均明显减少,HBO组均明显增加(F=88.692,P<0.01)。结论大鼠少突胶质细胞前体细胞ADAM10分子在一氧化碳中毒造成的脑白质损伤及高压氧治疗中发挥重要作用。Objective To investigate the effect of hyperbaric oxygen （HBO） treatment on the xpression of Oligodendro- cyte Precursors （OPCs） ADAM10 mRNA in the acute carbon monoxide poisoning（ACOP） rats. Methods OPCs ceils of neonatal rat were cultured in vitro and divided into three groups： control, ACOP and HBO groups. Control group： no treatment, ACOP group： the OPC cells were placed in a sealed container and injected with 1% carbon monoxide ac cording to the volume ratio, HBO group： OPCs cells after ACOP were placed in animal experiments of hyperbaric oxygen chamber, with 98% 02, 2%CO2 washing 10 rain, then 15 min 0.35 MPa pressure staying 2 h, at last 20 min reducing to normal pressure. The chamber temperature was controlled at 37~C. The end time of HBO treatment was set as 0 h, the three groups were collected at 6,24 h and 48 h for subsequent experiments. The expression of ADAM10 mRNA at 6, 24 h, and 48 h were detected by RT-PCR. Results 6, 24 h, and 48 h, the expression of ADAM10 mRNA was no change in control group, but gradually decreased in ACOP group, and increased in HBO group, there was statistically significant among the three groups （F = 8.491, P 〈 0.01）. Compared with control group, the expression of ADAMI0 mRNA at 6, 24, 48 h was significantly reduced in ACOP group, while was significantly increased in HBO group （F = 88.692, P 〈 0.01）. Conclusion ADAM10 of OPCs may play an important role in the injury of central nervous system after ACOP and HBO.

关 键 词：高压氧 一氧化碳中毒 少突胶质细胞前体细胞 ADAM10 

分 类 号：R595.1[医药卫生—内科学]