三种化学感受蛋白在意大利蜜蜂成年工蜂中的时空表达水平研究  

Spatiotemporal expression levels of three chemosensory proteins in adult Apis mellifera ligustica workers

作　　者：王宇飞[1] 施腾飞[1] 齐磊[2] 刘芳[2] 朱玉洁 侯超 余林生 

机构地区：[1]安徽农业大学植物保护学院,合肥230036 [2]安徽农业大学动物科技学院,合肥230036 [3]安徽省地方畜禽遗传与育种重点实验室,合肥230036

出　　处：《应用昆虫学报》2018年第1期71-78,共8页Chinese Journal of Applied Entomology

基　　金：国家蜂产业技术体系建设专项(CARS-45-KXJ9)

摘　　要：【目的】化学感受蛋白(Chemonsensory proteins,CSPs)是一类可溶性小分子蛋白质,广泛存在于昆虫的触角及其他感受器中,参与昆虫的化学感受行为,在昆虫化学感受系统中发挥重要的作用,本文旨在探究Amel-CSP1、Amel-CSP2和Amel-CSP6在意大利蜜蜂成年工蜂中的时空表达水平及生理功能。【方法】首先通过荧光定量PCR技术检测了Amel-CSP1、Amel-CSP2和Amel-CSP6在哺育蜂和采集蜂的触角、头部、胸部、腹部和腿的表达水平;然后又对不同日龄工蜂(1,6,12,18,28日龄)触角中Amel-CSP1、Amel-CSP2和Amel-CSP6的表达情况进行检测。【结果】(1)Amel-CSP1在工蜂的触角和头部都有较高的表达水平,其在工蜂触角中的表达水平显著高于其他部位(P<0.05);Amel-CSP2在哺育蜂触角中表达水平最高,显著高于其他部位(P<0.05),而其在采集蜂头部的表达水平最高,显著高于其他部位(P<0.05);Amel-CSP6在哺育蜂和采集蜂的头部表达量最高,其在触角、胸部也有较高表达水平。(2)Amel-CSP1在工蜂触角中的表达水平随日龄的增长而增加,其在28日龄的表达水平显著高于其他日龄(P<0.05);Amel-CSP2在1日龄和12日龄工蜂触角的表达量最高,显著高于其他日龄(P<0.05);Amel-CSP6在28日龄工蜂触角中的表达水平最低,显著低于其他日龄(P<0.05)。【结论】Amel-CSP1、Amel-CSP2、Amel-CSP6在意大利蜜蜂成年工蜂中的表达具有明显的时空特异性,这对进一步探测意大利蜜蜂CSPs家族的生理功能提供了研究方向和理论基础,具有一定的生物学意义。[Objectives] Chemosensory proteins （CSPs） are a kind of soluble small protein that is widespread in the antenna, and other chemical sensory organs, of insects. CSPs participate in the chemical behavior of insects and play a key role in chemoreception. We investigated the spatiotemporal expression levels, and explored the functions, of three CSPs; Amel-CSP1, Amel-CSP2 and Amel-CSP6, in adult Apis mellifera ligustica workers. [Methods] Expression levels of Amel-CSP1, Amel-CSP2 and Amel-CSP6 in different parts of nurse and forager bees, and in the antennae of 1, 6 12, 18 and 28-day-old workers, were quantified using RT-qPCR. [Results] （1） Amel-CSP1 was expressed to a high degree in the antennae and heads of adult workers, and its expression in antennae was significantly higher than in others body parts （P〈0.05）. Amel-CSP2 was expressed more highly in the antennae of nurses （P 〈 0.05）, whereas in foragers, expression of this gene was significantly higher in the head than in others organs （P 〈 0.05）. Amel-CSP6 was expressed most highly in the heads of nurses and foragers and also relatively highly in the antennae and thorax. （2） Expression of Amel-CSP1 increased with age, being significantly more highly expressed in 28-day-old workers （foragers） than in young workers （P 〈 0.05）. Amel-CSP2 was significantly more highly expressed in 1 and 12-day-old workers than in those of other ages （P 〈 0.05）. Expression of Amel-CSP6 was significantly lower in 28-day-old workers than in those of other ages （P 〈 0.05）. [Conclusion] The expression levels of Amel-CSPI, Amel-CSP2 and Amel-CSP6 all showed a high degree of spatiotemporal specificity in adult Apis mellifera ligustica workers. These results provide a theoretical basis for further exploration of the physiological functions of Amel-CSP1, Amel-CSP2 and Amel-CSP6, in A. m. ligustica.

关 键 词：意大利蜜蜂 工蜂 化学感受蛋白 时空表达 荧光定量PCR 

分 类 号：S891[农业科学—特种经济动物饲养]