Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid–liquid extraction system with ethanol and K_3PO_4  

作　　者：Daiana Wischral Hongxin Fu Fernando L.Pellegrini Pessoa Nei Pereira Jr Shang-Tian Yang 

机构地区：[1]School of Chemistry,Department of Biochemical Engineering,Federal University of Rio de Janeiro [2]William G.Lowrie Department of Chemical and Biomolecular Engineering,The Ohio State University [3]School of Chemistry,Department of Chemical Engineering,Federal University of Rio de Janeiro

出　　处：《Chinese Journal of Chemical Engineering》2018年第1期104-108,共5页中国化学工程学报（英文版）

基　　金：CNPq,FAPERJ and CAPES through the PDSE and Program and Human Resources Program 13 of the National Petroleum Agency (ANP-PRH 13)

摘　　要：1,3-Propanediol,traditionally obtained from fossils,has numerous industrial applications,including use in the production of high performance polymers.The microbial production of 1,3-propanediol presents several opportunities,and the final purity grade determines its price and commercial viability.The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol.Thus,we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth.Initially,a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology.The solvents studied were methanol,ethanol,isopropanol and acetone,and the salts examined were K_2HPO_4,Na_2CO_3,K_2CO_3,(NH_4)_2SO_4,NaHPO_4,K_3PO_4 and C_6H_5NaO_7.The optimal extraction system consisted of 34 wt%K_3PO_4,28 wt% ethanol,and 38 wt% fermentation broth containing 23.0 g·L^(-1)1,3-propanediol,which gave the highest partition coefficient of 33 and recovery yield of 97%.The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.1,3-Propanediol,traditionally obtained from fossils,has numerous industrial applications,including use in the production of high performance polymers.The microbial production of 1,3-propanediol presents several opportunities,and the final purity grade determines its price and commercial viability.The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol.Thus,we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth.Initially,a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology.The solvents studied were methanol,ethanol,isopropanol and acetone,and the salts examined were K2HPO4,Na2CO3,K2CO3,（NH4）2SO4,NaHPO4,K3PO4 and C6H5NaO7.The optimal extraction system consisted of 34 wt%K3PO4,28 wt% ethanol,and 38 wt% fermentation broth containing 23.0 g·L-11,3-propanediol,which gave the highest partition coefficient of 33 and recovery yield of 97%.The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.

关 键 词：Fermentation Downstream process 1 3-Propanediol Clostridium beijerinckii Liquid-liquid extraction Salting-out extraction 

分 类 号：TQ223.162[化学工程—有机化工]