川崎病并发冠脉损伤患儿血清活化HUVECs NF-κB促使MMP-9 mRNA水平上调的实验研究  

Experimental Study on the Up-Regulation of NF-kappa B and MMP-9 mRNA Level in HUVECs Stimulated by Serum from Children with Coronary Artery Lesions of Kawasaki Disease

作　　者：刘丽莎 李喆倩[1] 谈诚 于汉卿 LIU Li-sha;LI Zhe-qian;TAN Cheng;YU Han-qing(Department of Clinical Laboratory, Children＇s Hospital of Nanjing Medical University ,Nanjing 210008,China)

机构地区：[1]南京医科大学附属儿童医院检验科,南京210008

出　　处：《现代检验医学杂志》2018年第1期131-132,136,共3页Journal of Modern Laboratory Medicine

基　　金：南京市医学科技发展基金(YKK13131)

摘　　要：目的探讨脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)在川崎病(kawasaki disease,KD)并发冠状动脉损伤(coronary artery lesions,CALs)患儿血清作用下,核因子-κB(nuclear factor-kappa B,NF-κB)对基质金属蛋白酶9(matrix metalloproteases 9,MMP9)mRNA转录的影响。方法将HUVECs分为4组,分别为正常血清组(control组)、一般发热血清组(F组)、无冠脉损伤组(non-CALs组)和冠脉损伤组(CALs组)。免疫共沉淀(co-immunoprecipitation,ChIP)检测HUVECs中NF-κB p65与MMP-9启动子结合情况,RT-PCR检测HUVECs MMP-9mRNA水平。结果与对照组相比,经KD患儿,特别是并发CALs患儿血清作用后,HUVECs NF-κB p65可直接结合在MMP-9启动子上,同时MMP-9mRNA呈高表达。结论 HUVECs在KD并发CALs患儿血清作用下,NF-κB p65可直接结合在MMP-9启动子上促进其转录。Objective To investigate the relationship between nuclear factor-kappa B（NF-κB）and matrix metalloproteinase-9（MMP-9）mRNA in human umbilical vein endothelial cells（HUVECs）stimulated by the serum from children with coronary artery lesions of Kawasaki disease（KD）.Methods HUVECs were cultured and were divided into 4 groups：normal serum group,general fever group,Non-CALs group and CALs group.Co-Immunoprecipitation（ChIP）was used to detect the relationship between NF-κB and MMP-9,and RT-PCR was used to detect the mRNA level of MMP-9.Results Compared with control groups,NF-κB p65 could bind the promoter of MMP-9 in HUVECs cultured with 10%serum from KD patients with coronary artery lesions.The mRNA level of MMP-9 was also up-regulated.Conclusion NF-κB p65 can promote the transcription of MMP-9 in HUVECs induced by the serum from KD patients with coronary artery lesions.

关 键 词：川崎病 冠状动脉损伤 核因子-κB 基质金属蛋白酶9 

分 类 号：R725.5[医药卫生—儿科] R392.11[医药卫生—临床医学]