大豆分离蛋白/琼脂糖复合水凝胶  

Soy Protein Isolate/Agar Composite Hydrogel

作　　者：刘杰 周浩[2] 黄郁芳[3] 陈新[1] 

机构地区：[1]聚合物分子工程国家重点实验室,复旦大学高分子科学系,先进材料实验室,上海200433 [2]复旦大学附属眼耳鼻喉科医院,上海200031 [3]复旦大学材料科学系,上海200433

出　　处：《高等学校化学学报》2018年第3期591-597,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号：21274028,21574023,21574024)资助

摘　　要：采用简单的物理共混方法,制备一种大豆分离蛋白/琼脂糖降温型复合水凝胶.即在高温下聚乙二醇改性的大豆分离蛋白/琼脂糖混合物呈溶液状态,降温至生理温度37℃或更低温度则形成水凝胶.表征了该复合凝胶的溶胀度、力学性能、细胞毒性和细胞迁移率.结果表明,改性大豆分离蛋白/琼脂糖复合水凝胶具有较高的溶胀度和力学性能,良好的生物相容性和细胞迁移率,在伤口敷料领域显示出潜在的应用.Agar,a plant polysaccharide,was selected to form composite hydrogel with pure and polyethylene glycol（PEG）-modified soy protein isolates（SPI） by a simple blending method.It was found that SPI/agar mixture was in solution state at high temperature but quickly formed a hydrogel after cooling down to the physiological temperature（37 ℃） or lower.Therefore,such a natural polymer-based thermo-sensitive hydrogel can be designed into different shapes.SEM images indicated that PEG-modified SPI/agar hydrogel had more compact network structure than the pure SPI/agar hydrogel.The swelling ratio,mechanical properties,cytotoxicity,and cell migration ability of both pure and PEG-modified SPI/agar composite hydrogel were extensively investigated.Compared with the pure agar hydrogel and pure SPI/agar composite hydrogel,PEG-modified SPI/agar hydrogel showed the largest swelling ratio（higher than 1600%）.In addition,the PEG-modified SPI/agar composite hydrogels showed good mechanical properties,which was comparable to that of pure agar hydrogel.In vitro cell experiments demonstrated that PEG-modified SPI/agar hydrogel had both good biocompatibility and high cell migration ability.Therefore,all excellent properties shown above made the PEG-modified SPI/agar hydrogel a good candidate for wound dressing.

关 键 词：改性大豆分离蛋白 琼脂糖 复合水凝胶 伤口敷料 

分 类 号：O636[理学—高分子化学]