NOG基因与不明原因自然流产相关性的初步探讨  

Study on the correlation between NOG genetic and unexplained miscarrige

作　　者：薛慧琴 周岩 卢洪涌 孙夏瑜 张玉萍 王丽燕 燕美琴 

机构地区：[1]山西省儿童医院山西省妇幼保健院,太原030013

出　　处：《中国优生与遗传杂志》2018年第2期71-73,共3页Chinese Journal of Birth Health & Heredity

基　　金：山西省儿童医院山西省妇幼保健院博士基金项目(编号:201509)

摘　　要：目的研究包含NOG基因的TGF-β/BMP信号通路在不明原因自然流产中的作用和可能的作用机制。方法用TGF-β/BMP信号通路基因芯片检测了自然流产和人工流产胚胎组织的m RNA表达谱的差异,差异最大的NOG基因进一步用Western-blot检测了实验组和对照组蛋白表达水平的差异。结果两组之间有24个基因存在差异性表达,进一步进行功能研究发现,NOG基因作为BMP配体的抑制因子,在蛋白水平也存在显著差异(P<0.05)。结论 NOG基因的表达上调与自然流产有关,可能的机制为NOG基因表达上调引起了TGF-β/BMP信号通路功能失调参与了不明原因自然流产的发生。Objective：The aim of this study was to investigate the function and underlying mechanism of TGF-β/BMP signaling pathway in early unexplained miscarriage. Methods：Expression profiles of genes involved in TGF-β/BMP signaling pathway,which presented in the Human TGF β/BMP Signaling Pathway RT^2 ProfilerTM PCR Array,were compared between placental villous tissue samples from 15 women with missed abortion and those from 15 women with induced abortion. The protein expression level of the most upregulated gene NOG was further measured using western blotting in another 15 women with missed abortion and 15 women with induced abortion. Results：There are in total 24 genes showed differential expression level between the two groups. Their functions were further investigated. Surprisingly,it was found that up to 6 of 13 upregulated genes were TGF-β responsive genes. The most increased gene is NOG,an antagonist of TGF-β ligand. The protein expression level of NOG was confirmed to show the same trend as microarray using western blotting. Conclusion：A increased expression of NOG in women with missed abortion was identified compare with women with induced abortion,which may result in a dysregulation of TGF-βsignaling and may be the underlying mechanism of missed abortion.

关 键 词：自然流产 TGFβ/BMP信号通路 NOG基因 胚胎绒毛组织 

分 类 号：R714.21[医药卫生—妇产科学]