新型可降解镁锌合金材料应用于动静脉瘘封堵材料的动物研究  

Animal experimental study of new biodegradable Mg-Zn alloy occluder implanted in aortic-vena cava fistula

作　　者：杨文超[1] 赵珺[1] 梅家才[1] 邵明哲[1] 潘烨[1] 张健[1] 吴海生[1] 汪涛[1] 于敏[1] 蒋玉洁[1] 黄月亭 赵常利[2] 张小农[2] 

机构地区：[1]上海交通大学附属第六人民医院血管外科,上海200233 [2]上海交通大学材料科学与工程学院,上海200240

出　　处：《中国血管外科杂志（电子版）》2017年第4期269-273,共5页Chinese Journal of Vascular Surgery(Electronic Version)

摘　　要：目的构建犬动静脉瘘动物模型,对新型可降解镁锌合金材料植入动静脉瘘动物模型后生物相容性及安全性进行研究。方法实验用比格犬10只,构建犬髂内动静脉瘘动物模型,分为实验组(镁锌合金材料植入动静脉瘘)5只和对照组(仅动静脉瘘造模)5只,比较两组实验动物饮食、活动及切口反应情况,分别于术前及术后1、4、8及12周检测血清肝肾功电解质(肌酐、谷丙转氨酶、总胆红素、尿素氮及血镁锌浓度)生化指标,按照计划在术后12周处死实验犬,取出动静脉瘘标本及肺肝肾心等重要脏器进行苏木精-伊红(HE)染色。结果两组实验动物饮食、活动正常、切口未见明显感染迹象。术前及术后谷丙转氨酶、总胆红素及尿素氮浓度差异无统计学意义(P>0.05);血镁浓度在术后1周、血锌及肌酐浓度在术后1、4周差异有统计学意义(P<0.05),而其余时间点血镁锌及肌酐浓度差异无统计学意义(P>0.05)。术后12周HE染色示实验组动静脉瘘处动脉内膜较对照组稍增生,未发现明显炎症细胞浸润;肾小管和间质未出现异常形态、肿胀及明显炎症反应;肺泡结构和功能完整,未发现明显血栓形成;肝、心细胞未出现水肿及坏死。结论新型可降解镁锌合金材料植入犬动静脉模型后,对血液生化指标及重要脏器结构功能无明显影响,可作为动静脉瘘的理想封堵材料,具有良好的前景及临床应用价值。Objective To evaluate the biocompatibility and safety of the new biodegradable Mg-Zn alloy used as occluder materials in dogs with aortic-vena cava fistula. Methods Ten beagle dogs were used to establish aortic-vena cava fistula model. They were equally divided into experimental group(MgZn alloy implanted aortic-vena cava fistula)(n=5) and control group(noting implanted aortic-vena cava fistula)(n=5). The diet, activity and incision response of dogs were observed. Pre-and post-operationally,the blood biochemical test, serum magnesium and zinc were compared between the two groups. After 12 weeks, all beagles were executed by lethal dose of pentobarbital after removal of the heart, liver, lung,kidney and roughly 5 cm of aortic-vena cava fistula specimen adopting by hematoxylin and eosin(HE)staining. Results The diet and activity of experimental animals between two groups were normal, and incision was not obvious signs of infection. Pre-and post-operationally, indicators such as ALT 、TBi L and BUN were found no statistically significant differences in the experimental group compared to the control group at each time point(P >0.05). Although serum magnesium values in the experimental group in the first week were gently higher than the control group(P <0.05), no difference was discovered at other time point(P >0.05). The serum zinc Scr levels in the experimental group were higher than the control group in the first and fourth week respectively(P<0.05), but there were no statistically significant differences at other time point(P>0.05). Though HE staining assessment, all aortic-vena cava fistula specimens manifested no significant inflammatory response with slightly thickened intima, without obvious inflammatory cell infiltration. The tubules and interstitium of kidney did not present abnormal morphology, without swelling or inflammatory reaction. The structure and function of alveoli was complete and no PE was observed. Hepatocytes did not show obvious edema or necrosis. Apoptosis or necrosis of myocardial cell was

关 键 词：镁锌合金 可降解 动静脉瘘 生物相容性 安全性 动物模型 

分 类 号：R318.08[医药卫生—生物医学工程] R654[医药卫生—基础医学]