杜鹃炭疽病病原鉴定及其生物学特性研究  

Pathogen Identification and Biological Characteristics of Rhododendron Anthracnose

作　　者：杨秀梅[1] 唐艺榕 李进昆[1] 龙江[1] 解玮佳[1] 周旭红[1] 

机构地区：[1]云南省农业科学院花卉研究所/云南省花卉育种重点实验室/国家观赏园艺工程技术研究中心,云南昆明650205

出　　处：《江西农业学报》2018年第3期74-77,82,共5页Acta Agriculturae Jiangxi

基　　金：云南省花卉育种重点实验室建设专项(2015DG013)

摘　　要：从昆明市郊的杜鹃盆花种植基地采集炭疽病样品,经病原分离和纯化得到形态一致的6个菌株;通过形态学观察、分子生物学和致病性鉴定,将该杜鹃炭疽病的病原菌鉴定为博宁炭疽菌(Colletotrichum boninense)。研究了不同碳源、氮源、p H值、温度对该病原菌生长的影响,结果表明:该病原菌能有效利用多种碳源,最适合的为葡萄糖;有利于该病原菌生长的有机氮为蛋白胨,无机氮为硝酸钾;病原菌在15~35℃下均能生长,最适温度为25℃;适于病原菌生长的培养基p H值为6~8;适于产孢的培养基为MA培养基。The rhododendron anthracnose samples were collected from the planting base of rhododendron pot-flower in thesuburb of Kunming city, and six strains with the same morphological characteristics were obtained through pathogen isolation andpurification. These strains were identified as Colletotrichum boninense based on morphological observation, molecular biologicalcharacteristics and pathogenicity test. The effects of carbon source, nitrogen source, pH-value, and culture temperature on thegrowth of this pathogen were studied. The results showed that： this pathogen could effectively utilize many kinds of carbonsources, and the best carbon source was glucose; the optimal organic and inorganic nitrogen sources were peptone and potassiumnitrate, respectively; the optimum culture temperature was 25 ℃; the best pH-value of medium for the growth of C. boninensewas 6 ～ 8; the suitable medium for spore formation was MA medium.

关 键 词：杜鹃 炭疽病 病原鉴定 生物学特性 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]