桑椹中新绿原酸、绿原酸、芦丁和异槲皮苷的含量测定  

Determination of Neochlorogenic Acid,Chlorogenic Acid,Rutin and Isoquercitrin in Fructus Mori

作　　者：陈志永 杨媛媛[2] 蒙麦侠 杨智峰[1] 李晔[1] 张紫佳[2] 孟雪[1] 

机构地区：[1]陕西省中医药研究院,陕西西安710003 [2]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201203

出　　处：《辽宁中医杂志》2018年第1期116-118,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81603264)

摘　　要：目的：建立同时测定桑椹中新绿原酸、绿原酸、芦丁和异槲皮苷4种有效成分含量的准确、精密的高效液相色谱方法。方法：实验中采用Syncronis a Q-C18（Thermo scientific;4.6 mm×250 mm,5μm）色谱柱,乙腈-0.1%甲酸水作流动相,梯度洗脱,流速1 m L·min-1,检测波长350 nm。结果：新绿原酸、绿原酸、芦丁和异槲皮苷在质量浓度分别为0.00106~0.0424 mg/m L,0.00794~0.318 mg/m L,0.00214~0.0856 mg/m L,0.00136~0.0544 mg/m L范围内与峰面积呈良好的线性关系（r〉0.9996）;4种有效成分的平均回收率分别为97.7%,99.4%,97.4%,100.4%,RSD分别为1.39%,2.09%,0.62%,1.62%。结论：该方法简便、准确,分离效果好,可用于桑椹的质量评价。Objective： To establish an accurate and reliable analytical method for the simultaneous determination of four active components（neochlorogenic acid,chlorogenic acid,rutin and isoquercitrin） in Fructus Mori. Methods： Neochlorogenic acid,chlorogenic acid,rutin and isoquercitrin in the samples were isolated by gradual elution with acetonitrile-0. 1% formic acid solution and detected by HPLC. The chromatographic condictions： Syncronisa Q-C18（Thermo scientific,4. 6 mm × 250 mm,5 μm） column,flowing rate being 1 m L·min-1,detecting wavelength at 350 nm. Results： Good linearities of neochlorogenic acid,chlorogenic acid,rutin and isoquercitrin were in the range of 0. 00106-0. 0424 mg/m L,0. 00794-0. 318 mg/m L,0. 00214-0. 0856 mg/m L,0. 00136-0. 0544 mg/m L,respectively（r 0. 9996）. The average recoveries of the four components were 97. 7%,99. 4%,97. 4% and 100. 4%. The relative standard deviation was 1. 39%,2. 09%,0. 62% and 1. 62%. Conclusion： The method is simple,feasible and reproducible and can be used for the quality control of Fructus Mori.

关 键 词：桑椹 含量测定 新绿原酸 绿原酸 芦丁 异槲皮苷 

分 类 号：R284[医药卫生—中药学]