HPLC法测定苓桂术甘汤中五种成分的含量  

Simultaneous Determination of Five Active Ingredients in Linggui Zhugan Decoction by HPLC

作　　者：胡谦锋 申士富[2] 石银龙 王一[1] 周春祥[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210000 [2]南京中医药大学药学院,江苏南京210000

出　　处：《辽宁中医杂志》2018年第1期126-130,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81503485);国家自然科学基金面上项目(81774021)

摘　　要：目的：建立HPLC法同时测定苓桂术甘汤中肉桂酸、桂皮醛、甘草酸、白术内酯Ⅲ、茯苓酸的量。方法：采用XBridge TM（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-0.1%磷酸溶液梯度洗脱,体积流量为0.8 m L/min,检测波长为210 nm,柱温为30℃。结果：肉桂酸、桂皮醛、甘草酸、白术内酯Ⅲ、茯苓酸5种成分在100 min内被完全分离;峰面积与其浓度成良好的线性关系;加样回收率（n=6）为99.39%~100.06%（RSD=0.22%~2.19%）。结论：采用高效液相色谱法同时测定复方中的5种成分,此方法简单可靠,精密度好,可较好地用于苓桂术甘汤的质量控制。Objective： To establish an HPLC method to simultaneously determine the amount of guangxi cinnamic acid,cinnamaldehyde,glycyrrhizic acid,atractylodes lactone Ⅲ and poria cocos acid in Linggui Zhugan Decoction. Methods： The XBridge TM（250 mm × 4. 6 mm,5 microns） chromatographic column was used and the mobile phase gradient elution was 0. 1% acetonitrile-phosphate solution. The volume flow was 0. 8 m L/min and the detection wavelength was 210 nm. The column temperature was30 ℃. Results： Cinnamic acid,cinnamaldehyde,glycyrrhizic acid,atractylodes lactone Ⅲ and poria cocos acid were completely separated in 100 minutes. Peak area and concentration had a good linear relationship. The sample recovery rate was 99. 39% ~100. 06%（n = 6）（RSD = 0. 22% ~ 0. 22%）. Conclusion： High performance liquid chromatography can determine the five elements at the same time. This method is simple and reliable with good precision and can be well used for quality control of Linggui Zhugan Decoction.

关 键 词：高效液相色谱法 苓桂术甘汤 定量测定 肉桂酸 桂皮醛 甘草酸 白术内酯Ⅲ 茯苓酸 

分 类 号：R284[医药卫生—中药学]