骨碎补总黄酮对大鼠胫骨牵张末期BMP-2与Smad-1表达的影响  

Effects of Assemble Flavone of Drynaria Rhizome on BMP-2 and Smad-1 Expressions in Rat Tibia During the Last Stage of Distraction Osteogenesis

作　　者：姜自伟[1] 曾景奇[1] 李悦[1] 黄枫[1] 王凡 余翔[1] 朱海峰[1] 

机构地区：[1]广州中医药大学第一附属医院

出　　处：《辽宁中医杂志》2018年第1期166-168,I0007,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81403413)

摘　　要：目的：探讨骨碎补总黄酮干预下BMP-2与Smad-1在大鼠胫骨牵张成骨末期中的表达。方法：40只大鼠建立大鼠胫骨牵张成骨模型,随机分成4组,分别为对照组、中药组、阻遏剂组及阻遏剂加中药组,每组10只。所有大鼠于牵张第20天时给予拍片评估,处死,取标本,HE染色评价成骨质量,用免疫组化分析各组BMP-2与Smad-1的表达。结果：中药组BMP-2与Smad-1的表达最高（P〈0.05）,阻遏剂加中药组明显高于阻遏剂组（P〈0.05）。结论：BMP-2与Smad-1的表达在牵张成骨期间对成骨有重要促进作用,补肾法可以促进大鼠胫骨牵张末期BMP-2与Smad-1的表达。Objective： To explore the expressions of BMP-2 and Smad-1 in rat tibia which were treated by drynaria rhizome during distraction osteogenesis. Methods： Forty rat tibia distraction osteogenesis models were constructed,then were randomly assigned into four groups,which were control group,Chinese medicines group,Suppressor group and Suppressor adding Chinese medicine group,there were ten animals in each group. All animals were examined with X-Ray on 20 th day since distraction,then were sacrificed for tissues sampling and trimming. HE stanning was conducted to evaluate osteogenesis quality,and Immunohistochemistry was used to estimate the expressions of BMP-2 and Smad-1. Results： The expression levels of BMP-2 and Smad-1 were the highest in Chinese medicine group（P 0. 05）,and which were higher in Suppressor adding Chinese medicine group than that in Suppressor group. Conclusion： The expressions of BMP-2 and Smad-1 have important facilitation on osteogenesis during distraction osteogenesis,and the expressions of BMP-2 and Smad-1 during the last stage of distraction osteogenesis could be promoted by tonifying kidney method.

关 键 词：骨碎补总黄酮 BMP-2 Smad-1 牵张成骨 

分 类 号：R285.5[医药卫生—中药学]