剑叶龙血树化学诱导结血物中龙血素A、龙血素B的测定及诱导剂效果评价  

Determination of Loureirin A and Loureirin B in Artificially Induced Dragon's Blood of Dracaena cochinchinensis

作　　者：陈乾平[1] 唐春风[1] 龙海荣[1] 何丽丽[1] 

机构地区：[1]广西壮族自治区药用植物园,广西南宁530023

出　　处：《当代化工》2018年第2期434-436,440,共4页Contemporary Chemical Industry

基　　金：广西自然科学基金;项目号:2014GXNSFBA118067

摘　　要：目的建立高效液相测定剑叶龙血树人工诱导结血物中龙血素A、B的方法,用于筛选有效的剑叶龙血树结血诱导剂。方法高效液相色谱法(HPLC)法,色谱柱安捷伦ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5μm),流动相为乙腈-1%冰醋酸(40∶60),流速1.0 m L/min,柱温25℃,检测波长为280 nm。结果龙血素A在0.021 6~0.194 8μg范围内呈线性关系,回归方程为Y=-0.783 6X+1.825 1,r=0.999 8,龙血素B在0.020 1~0.181 1μg范围内呈线性关系,回归方程为Y=-0.478 5X+1.054 8,r=0.999 7,不同诱导剂诱导所得龙血素A含量在0.013%~0.099%,龙血素B含量在0.044%~0.097%。结论该方法快速、准确,可用于剑叶龙血树人工诱导结血物中龙血素A、B的检测,茉莉酸甲酯(50 mg/L)-苯甲酸(2 g/L)、硫酸镁(5%)-苯甲酸(2 g/L)、3吲哚丁酸(5 mg/L)-苯甲酸(2 g/L)、的诱导结血效果较好。A determination method of Loureirin A and Loureirin B in artificially induced dragon＇s blood of Dracaena cochinchinensis by HPLC was established to screen effective inducer. The Loureirin A and Loureirin B were separated on Agilent ZORBAX Eclipse XDB C18（4.6 mm×250 mm,5μm）. UV detection wavelength was at 280 nm. The results indicated that the Loureirin A content showed good linear in the range of 0.021 6~0.194 8 μg,regression equation： Y=-0.783 6 X＋1.825 1,correlation coefficient（r）=0.999 8;The Loureirin B content showed good linear in the range of 0.0201~0.1811 μg,regression equation： Y=-0.4785 X＋1.054 8,correlation coefficient（r）=0.999 7.The content of Loureirin A ranged from 0.013% to 0.099% and the content of Loureirin B ranged from 0.044% to 0.097%.The determination method of Loureirin A and Loureirin B in artificially induced dragon＇s blood of Dracaena cochinchinensis is simple and repeatable, and can be used for screening the effective inducer of dragon＇s blood of Dracaena cochinchinensis.

关 键 词：龙血竭 龙血素A 龙血素B 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]