用RISA法评估补饲对枯草期放牧羊细菌群落结构的影响  

Evaluation of Supplementary Feeding in Grazing Sheep Bacterial Communities by Using Ribosomal Intergenic Spacer Analysis(RISA)

作　　者：李长青 金海 薛树媛 郭天龙 

机构地区：[1]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《家畜生态学报》2016年第2期40-44,共5页Journal of Domestic Animal Ecology

基　　金：内蒙古自然科学基金资助项目(2012MS0417);现代肉羊产业技术体系建设专项资金资助(CARS-39)

摘　　要：为了揭示补饲不同精料对放牧羊瘤胃微生物多样性及瘤胃微生物群落结构的影响,为放牧羊的科学补饲提供基础资料,本研究以6只杜蒙杂交羔羊为研究对象,运用RISA法对补饲不同精料的放牧羊瘤胃微生物多样性及瘤胃微生物群落结构进行了分析。结果表明,放牧羊在补饲不同蛋白水平的精料后,瘤胃微生物多样性明显提高,体重显著增加(P<0.01)。放牧羊瘤胃微生物主要以硬壁菌门、拟杆菌门为主,Prevotella和Selenomonas ruminantium是瘤胃中分布数量最多的细菌,与瘤胃发酵密切相关。To investigate rumen microbial diversity and bacterial communities of grazing sheep supplemented with feeding,and provide the basic research data for sheep supplementary,six grazing lambs were taken as the research objects,and ribosomal intergenic spacer analysis(RISA)was applied to analyze rumen microbial diversity and bacterial communities.The results show that the microbial diversity and weight gain increased significantly(P<0.01)after sheep supplemented with different protein levels feeding.Firmicutes and Bacteroidetes are the dominant bacteria in rumen.Prevotella and Selenomonas ruminantium is the largest number in the rumen,and it is closely related with rumen fermentation.

关 键 词：RISA 补饲 放牧羊 细菌群落 

分 类 号：S826[农业科学—畜牧学]