电针不同腧穴配伍对心肌缺血损伤大鼠心肌功能的影响  

The effect of different acupoints of electroacupuncture on myocardial function in rats with myocardial ischemia injury

作　　者：张丽[1] 艾霞 彭页[3] 刘宝玉[4] 

机构地区：[1]宝鸡职业技术学院中医药学系,陕西宝鸡721013 [2]陕西中医药大学针灸推拿学院,陕西咸阳712046 [3]解放军空军总医院骨科,北京100142 [4]陕西省宝鸡市中医医院骨伤二科,陕西宝鸡721001

出　　处：《中国医药导报》2018年第3期134-137,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81403451)

摘　　要：目的探讨电针不同腧穴配伍对心肌缺血损伤大鼠心肌功能的影响。方法按随机数字表法将60只雄性Wistar大鼠分为正常组、模型组、腧穴配伍组、常规配穴组四组,每组各15只。造模方法 :盐酸异丙肾上腺素皮下注射(5 m L/kg)。正常组注射同等剂量的0.9%氯化钠溶液,1次/d,连续1周;模型组造模;腧穴配伍组取造模成功的大鼠,电针其双内关穴、双足三里穴及关元穴,每天各1次,连续2周;常规配穴组取双内关、双神门及膻中,余法同腧穴配伍组。观察各组大鼠心电图变化及心肌超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果与正常组比较,模型组心率(HR)加快明显,S-T段振幅抬高显著,差异有高度统计学意义(P<0.01);电针干预后,腧穴配伍组及常规配穴组HR有所减慢,S-T段振幅抬高降低,与模型组比较,差异有统计学意义(P<0.05)。与正常组比较,模型组大鼠心肌SOD活性、GSH-Px活性显著降低,MDA含量显著升高,差异有高度统计学意义(P<0.01);电针干预后,腧穴配伍组及常规配穴组大鼠心肌SOD活性、GSHPx活性显著升高,MDA含量降低,与模型组比较,差异有统计学意义(P<0.05);与常规配穴组比较,腧穴配伍组心肌SOD、GSH-Px活性升高,MDA含量降低,差异有统计学意义(P<0.05)。结论腧穴配伍针刺法具有很好的保护心肌缺血的效果,能够有效减轻心肌细胞变性及心肌组织的损害,为临床运用针灸治疗心肌缺血性疾病提供依据。Objective To explore the effect of different acupoints of electroacupuncture on myocardial function in rats with myocardial ischemia injury. Methods Sixty male Wistar rats were randomly divided into four groups by random number table method： normal group, model group, acupoint compatibility group, normal distribution group, with 15 rats in each group. Building methods： subcutaneous injection of Isoproterenol Hydrochloride（5 mL/kg） was given. Normal group： the same dose of 0.9% sodium chloride solution was injected, once a day, for one week. Model group was made the model. Acupoint compatibility group： the successful rats of the model were taken, and each day, the electric needle of the double ＂neiguan＂, double ＂zusanli＂ and ＂guanyuan＂ were performed respectively for two weeks. Normal distribution group： ＂neiguan＂, double ＂shenmen＂ and ＂danzhong＂ were pe rformed respectively, the followed method was as the same as the acupoint compatibility group. The changes of electrocardiogram in rats, and the activities of superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） and malondialdehyde（MDA） were observed. Results Compared with normal group, the heart rate（HR） in model group was accelerated significantly, and the amplitude of S-T segment was significantly enhanced, the differences were highly statistically significant（P〈0.01）. After the electroacupuncture intervention, the HR of the acupoints in the acupoint compatibility group and normal distribution group was decreased,and the amplitude of S-T was decreased, which had statistically significant differences compared with those of model group（P〈0.05）. Compared with the normalgroup, the activities of myocardial SOD and GSH-Px in the model group were decreased significantly, and the content of MDA was increased significantly, the differences were highly statistically significant（P〈0.01）. After the electroacupuncture intervention, the activities of SOD and GSH-Px of the myocardium of the rats in t

关 键 词：腧穴配伍 电针 心肌缺血损伤 心肌功能 

分 类 号：R245[医药卫生—针灸推拿学]