杠柳苷元大鼠在体肠吸收动力学研究  

Studies on Absorption Kinetics of Periplogenin in Rat Intestine

作　　者：尹通[1,2,3] 王晓明 潘桂湘[1,2] 郭亚卿 王献瑞[1,2,3] 吕弘 

机构地区：[1]天津中医药大学,天津300193 [2]天津市现代中药重点实验室-省部共建国家重点实验室(培育),天津300193 [3]天津国际生物医药联合研究院,天津300457

出　　处：《辽宁中医药大学学报》2018年第2期21-24,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB723504);国家科技支撑计划资助项目(2011BAI07B08)

摘　　要：目的：考察杠柳苷元大鼠在体肠吸收动力学,探讨杠柳苷元可能的吸收机制。方法：采用大鼠在体单向肠灌流模型,使用高效液相色谱法以Hypersil ODS2（4.6 mm×250 mm,5μm）色谱柱为固定相,乙腈-水（29∶71）为流动相,流速1 mL·min^（-1）,于220 nm波长测定灌流液中杠柳苷元的含量。结果：杠柳苷元不同浓度下的吸收速率常数（Ka）和表观吸收系数（Papp）在考察浓度范围内（1.25、5、20μg·mL^（-1））具有显著性差异（P〈0.01）,存在浓度依赖性。结论：杠柳苷元的肠吸收机制除被动扩散外,可能还涉及主动转运。Objective：To clarify the intestinal absorption characteristics of periplogenin in rats.Methods：With the in situ rat single-pass intestinal perfusion model,the perfusate samples were protein precipitated with methanol.After sample preparation,the samples were separated on an Elite Hypersil ODS2（4.6 mm×250 mm,5 μm）column and detected at 220 nm using acetonitrile and water（29∶71）as mobile phase.HPLC method was used to determine the concentrations of periplogenin in perfusion fluid.Results：The absorption rat constant（Ka）and apparent absorption coefficient（Papp）of periplogenin showed significant difference（P0.01）at different concentrations（1.25,5,20 μg·mL^（-1））in a concentrationdependent manner.Conclusion：In addition to the passive diffusion,the active transport may be one of mechanisms of the intestinal absorption of periplogenin.

关 键 词：杠柳苷元 在体肠吸收 单向肠灌流模型 被动扩散 主动转运 

分 类 号：R284[医药卫生—中药学]