“软坚护肝片”含药血清对Hep-G2细胞增殖及凋亡影响  

Effects of Ruanjian Hugan Tablet on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell Hep-G2

作　　者：孙振 李书[2] 陈兴东 李茜 罗冰 周晓东 

机构地区：[1]南京总医院疗养区,江苏南京211131 [2]上海中医药大学附属曙光医院宝山分院,上海201900

出　　处：《辽宁中医药大学学报》2018年第2期24-26,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81603458);南京总医院军事医学课题项目(YYMS2014013);南京总医院院管课题项目(2015068);南京总医院院管课题项目(2016083);江苏省药学会-奥赛康医院药学科研项目(201521);中国博士后科学基金面上资助项目(2012M521937)

摘　　要：目的：探讨＂软坚护肝片＂含药血清对Hep-G2细胞增殖和凋亡的影响。方法：给予人肝癌Hep-G2细胞不同剂量浓度的软坚护肝片含药血清进行干预。MTT法检测软坚护肝片对肝癌Hep-G2细胞增殖的影响;流式细胞术检测软坚护肝片对Hep-G2细胞周期、凋亡的作用;应用Western-blot法检测相关凋亡蛋白Bcl-2的变化。结果：软坚护肝片能抑制肝癌细胞增殖、诱导肝癌凋亡,其作用具有剂量依赖性,进一步研究显示,软坚护肝片可上调促凋亡蛋白Bax表达,抑制抗凋亡蛋白Bcl-2表达。结论：研究表明软坚护肝片含药血清能够诱导肝癌细胞凋亡,但对肝癌细胞周期分布无显著影响;且能上调促凋亡蛋白Bax表达,抑制抗凋亡蛋白Bcl-2表达。Objective：We investigated the mechanisms of the anti-hepatocellular carcinoma（HCC）effect of Ruanjian Hugan（RJHG）Tablet.Methods：The effect of RJHG tablet-medicated serum on the growth of Hep-G2 cells was evaluated by MTT assay in vitro.The effect of RJHG tablet-medicated serum on the cell cycle and apoptosis of Hep-G2 cells was analyzed by flow cytometry.The apoptosis related proteins,Bcl-2 and Bax was determined using Western blot.Results：RJHG tablet-medicated serum significantly suppressed the growth of Hep-G2 cells in a time-and dose-dependent manner.Treatment with RJHG tablet-medicated serum for 24 h increased the apoptosis rate of Hep-G2 cells,but did not alter the cell cycle distribution.RJHG tablet-medicated serum significantly downregulated the expression of Bcl-2 and upregulated the expression of Bax.Conclusion：In conclusion,our data suggest that RJHG tablet suppressed Hep-G2 cells proliferation by inducing cells apoptosis,but not cell cycle arrest through altering the expression of Bcl-2 and Bax.

关 键 词：凋亡 软坚护肝片 中医药 肝癌 

分 类 号：R285.5[医药卫生—中药学]