有机磷农药废水高效降解菌的筛选及菌群构建  

Screening of Highly Efficient Degradation Strain and Mixed Flora for Organophosphorus Pesticide Wastewater

作　　者：李铁军[1] 丁邦琴[1] 周春晓[1] 李德全[2] 

机构地区：[1]南通职业大学化学与生物工程学院,江苏南通226007 [2]南通大学生命科学学院,江苏南通226007

出　　处：《湖北农业科学》2018年第3期19-22,26,共5页Hubei Agricultural Sciences

基　　金：江苏省"十二五"高等学校重点专业(高职高专)应用化工技术群建设项目;南通市市级科技计划应用基础研究项目(MS12015036);南通市市级科技计划应用基础研究项目(GY12015027);南通职业大学高等教育教改重点研究课题(2015-ZD-07)

摘　　要：利用有机磷农药废水培养基,从江苏省南通市有机磷农药生产企业污水处理池活性污泥中驯化、分离了52株具有有机磷农药废水降解能力的菌种,建立资源库。对菌种资源库进行高效有机磷农药废水降解菌的筛选,获得14株72 h COD去除率大于60%的菌株。经形态学观察和16S rDNA初步鉴定,10株细菌分属于假单胞菌属(Pesudomonas)、不动菌属(Acinetobacter)、芽孢杆菌属(Bacillus)、副球菌属(Paracoccus);4株真菌分属于丝孢酵母属(Trichosporon)、曲霉属(Aspergillus)、木霉属(Trichoderma)、青霉属(Piniciellum)。选择降解率高的5株菌进行组合培养,筛选了6组72 h COD去除率大于70%的高效降解有机磷农药废水的菌群组合。Based on medium made of organic phosphorus pesticide wastewater,a resource library contains 52 strains with degrading ability of organophosphorus pesticide wastewater was set up, these strains were domesticated and isolated from activated sludge from sewage treatment tank of organophosphorus pesticide production enterprises in Nantong city,Jiangsu province.14 strains with 72 h COD removal rate more than 60% were obtained by screening of highly efficient degradation strain from the library. Through morphological observation and 16S rDNA identification,10 strains of bacteria belong to Pseudomonas,Acinetobacter,Bacillus,and the genus Paracoccus,and 4 fungi were classified as Trichosporon,Aspergillus,Trichoderma and Penicillium. Finally,6 group of flora combination with 72 h COD removal rate more than 70% were selected by using 5 strains with high degradation rate.

关 键 词：有机磷农药废水 高效降解菌 菌群组合 筛选 

分 类 号：X592[环境科学与工程—环境工程]