p11对神经细胞自噬的影响及其作用机制  

The Role of p11 in Nerve Cells Autophagy and Its Underlying Mechanism

作　　者：冯来鹏 郭继强[1,2,3] 杨婷婷 沈颖 王辉 

机构地区：[1]新乡医学院免疫学研究中心,新乡453000 [2]河南省分子诊断与医学检验技术协同创新中心,新乡453000 [3]河南省免疫与靶向药物重点实验室,新乡453000

出　　处：《中国细胞生物学学报》2018年第1期82-88,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:30670910);新乡医学院研究生科研创新支持计划项目(批准号:YJSCX201604Z)资助的课题~~

摘　　要：该研究主要探讨p11(又称为S100A10)蛋白质对神经母细胞瘤细胞系(SH-SY5Y)和小鼠海马细胞自噬的影响及其作用机制。该文利用p11过表达质粒pcDNA3.0-p11和p11基因敲低质粒Si-p11分别转染至SH-SY5Y内,24 h后提取其蛋白质和总RNA测其自噬相关基因的mRNA和蛋白质水平;利用C57BL/6J小鼠腹腔注射丙咪嗪,来上调小鼠海马p11的表达水平,然后提取其海马组织的蛋白质和总RNA测其自噬相关基因的表达情况。该研究结果显示,p11基因过表达对神经细胞自噬发挥下调作用,p11敲低后对自噬发挥上调作用;p11基因敲除小鼠的海马细胞的自噬是上调的,海马细胞p11高表达后自噬是下调的。因此认为,p11对于神经细胞自噬有可能发挥下调作用。This study is to explore the effect of p11（also known as S100 A10） on the autophagy of both neuroblastoma cell line（SH-SY5 Y） and mouse hippocampal cells and its mechanism. Both the plasmid of overexpression of p11（pcDNA3.0-p11） and the plasmid of gene knockdown of p11（Si-p11） were transfected into SHSY5 Y. Then after 24 h, total protein and RNA of SH-SY5 Y were extracted which were measured the expression of autophagy-related genes mRNAs and proteins. Then, C57 BL/6 J mice were injected imipramine into abdominal cavity to raise the expression level of p11 in hippocampus. The protein and RNA of hippocampus in injected C57 BL/6 J mice were extracted to detect the expression of autophagy-related genes. The results of the cell-experiments showed that the overexpression of p11 had a down-regulation effect on autophagy of nerve cells, and gene knockdown of p11 had an up-regulation effect on autophagy of nerve cells. The autophagy of hippocampal cells in mice without p11 was up-regulated, and the autophagy of hippocampal cells in mice with p11 overexpression was down-regulated. Therefore, we believe that p11 may play a down-regulated role in the autophagy of nerve cells.

关 键 词：基因敲低 转染 基因敲除 丙咪嗪 C57BL/6J 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]