CD11c^+DCs促K14-VEGF转基因小鼠银屑病样发病  

CD11c^+DCs promote the occurrence and development of psoriasis-like morbidity in K14-VEGF transgenic mice

作　　者：张怡 周彪[1] 郭政宏[1] 龙虎 严亨秀[1] 

机构地区：[1]西南民族大学,四川成都610041

出　　处：《基础医学与临床》2018年第2期205-212,共8页Basic and Clinical Medicine

基　　金：西南民族大学省级大学生创新创业训练计划(s201710656049);西南民族大学校级教改重大培育项目(2017ZDPY01);四川省教改项目"兽医专业学位研究生教育实践基地建设"(20140108)

摘　　要：目的研究CD11c^+DCs对银屑病的促进作用及机制。方法取K14-VEGF转基因小鼠皮损皮肤进行表型鉴定。将CD11c^+DCs注射入未发病的转基因小鼠颈部皮下,对小鼠进行PASI评分;组织学检测小鼠耳朵和颈背部皮肤;检测骨髓、血液、脾脏和颌下淋巴结处T淋巴细胞和DCs;免疫荧光法检测皮损中CD4、CD8、IL-17a、IFN-γ和CD31含量;HE染色方法观察小鼠心、肝、脾、肺和肾的变化。结果 K14-VEGF小鼠皮损中90%FLT3阳性细胞表达CD11c。实验组表皮层厚度均显著高于对照组(P<0.01)。血液中CD3^+CD4^+T淋巴细胞数降低(P<0.01);骨髓、血液、脾脏和颌下淋巴结中的Th1和Th17细胞减少;同时,骨髓和颌下淋巴结中的CD11c^+DCs减少(P<0.01);皮损皮肤中表达CD4^+T、CD8^+T、IL-17a^+和IFN-γ^+的细胞均增多(P<0.01),血管增多(P<0.01)。结论 CD11c^+DCs可通过促使K14-VEGF小鼠皮肤中T淋巴细胞及血管增多使该小鼠银屑病样病变提前发生,提示CD11c^+DCs可能在银屑病的发生发展中起重要作用。Objective To investigate the auxoaction and mechanism of CD11c^＋ DCs on psoriasis. Methods CD11c^＋ DCs in the psoriasis-like lesions were confirmed by immunofluorescence double staining experiments. The CD11c^＋ DCs were subcutaneously injected into the neck skin of transgenic mice without disease. The clinical features were observed and assessed with PASI score every day. The ear and back skin were checked by HE stained. At the same time,the T lymphocyte and DCs of bone marrow,spleen,submandibular lymph nodes and blood were analyzed by flow cytometry,and the T lymphocyte in the skin lesions were analyzed by immunofluorescence. The heart,liver,spleen,lungs and kindey were HE stained. Results In the psoriasis-like lesions,about 90% CD11c^＋ cells expressed CD11 c. HE test showed that the thickness of the skin layer was significant different between the experimental group（ ear： 29±4 μm; back： 25±3 μm） and the control group（ ear： 11±2 μm; back： 9±1 μm）（ P〈0. 01）.CD3^＋CD4^＋T lymphocyte cells in the blood of the experimental mice（ 17. 87 %） were decreased significantly（ P〈0. 01） compared with the control group mice（ 31. 77%）. The numbers of Th1 and Th17 cells from bone marrow,spleen,submaxillary lymph nodes and blood,as the same as CD11c^＋ DCs from bone marrow and submaxillary lymph nodes,were decreased in the experimental mice compared with the control group mice（ P〈0. 01）. The number of CD4^＋T cells,CD8^＋T cells,IL-17 a^＋cells and IFN-γ~＋cells in the skin lesions from the experimental group all were higher than that from the control group（ P〈0. 01）. And the vessels in the lesions of the experimental group were higher than that in the control group（ P〈0. 01）. Conclusions CD11c^＋ CD11c^＋ DCs may play an important role in the occurrence and development of psoriasis by increasing the T lymphocyte and the blood vessel in the skins of K14-VEGF transgenic mice.

关 键 词：CD11c^+DCs K14-VEGF转基因小鼠 银屑病 免疫调节 

分 类 号：R392.5[医药卫生—免疫学]