人端粒酶反转录酶过表达抑制低氧/复氧大鼠心肌细胞的凋亡  

Overexpression of human telomerase reverse transcriptase inhibits myocardial apoptosis induced by hypoxia/reoxigenation in rats

作　　者：王平善[1] 刘阳 

机构地区：[1]桂林医学院附属医院心外科,广西桂林541000

出　　处：《基础医学与临床》2018年第2期224-228,共5页Basic and Clinical Medicine

摘　　要：目的探讨人端粒酶反转录酶(TERT)在大鼠心肌低氧/复氧(Hypo/Ro)损伤中的保护作用。方法利用腺病毒将端粒酶催化亚基转染至大鼠心肌细胞;利用慢病毒介导小干扰核糖核酸(siRNA)沉默心肌细胞TERT基因;研究TERT过表达和沉默后对大鼠心肌细胞Hypo/Ro诱导的细胞凋亡的影响。结果 Hypo/Ro组细胞凋亡率显著高于对照组;TERT过表达组可显著降低Hypo/Ro心肌细胞的凋亡率;过表达的TERT可降低心肌细胞CytC的表达并增强P21的表达(P<0.05)。结论过表达的TERT可抑制Hypo/Ro诱导的心肌细胞凋亡,其机制可能与调控p21及CytC基因表达有关。Objective To investigate the effects of human telomerase reverse transcriptase（ TERT） on myocardial apoptosis induced by hypoxia/reoxigenation（ Hypo/Ro） in rats. Methods Adenovirus mediated transfection of TERT gene into myocardial cells of neonatal rats,lentivirus-mediated siRNA silencing the TERT. To investigate the protective effects of human telomerase reverse transcriptase on myocardial hypoxia/reoxigenation injury in rats.Results The apoptosis rate of Hypo/Ro group was significantly higher than that of the control group. Compared with the Hypo/Ro group,TERT over-expression group significantly reduced the rate of apoptosis,and over-expression of TERT decreased the expression of CytC gene and increased the expression of P21 gene in cardiomyo-cytes（ P〈0. 05）.Conclusions Over expression of telomerase reverse transcriptase inhibits myocardial apoptosis induced by Hypo/Ro. The mechanism may be explained by the regulation to the expression of P21 and CytC genes.

关 键 词：端粒酶反转录酶 心肌细胞 低氧/复氧损伤 

分 类 号：R541[医药卫生—心血管疾病]