鼻咽癌患者鼻咽灌洗液菌群检测及结果分析  

Detection and analysis of microbiota in nasopharyngeal lavage fluid in patients with nasopharyngeal carcinoma

作　　者：于湛[1] 邓晓琴[1] 邹杨[1] 吕秀鹏[1] 金肃年[1] 郭艳杰 李龙婕[1] 

机构地区：[1]大连医科大学附属第一医院肿瘤放疗科,辽宁大连116021 [2]大连医科大学基础医学院微生态学教研室,辽宁大连116044

出　　处：《中国微生态学杂志》2018年第2期137-140,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(81603423)

摘　　要：目的采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术,通过观察鼻咽癌患者与健康对照者鼻咽灌洗液菌群的变化,初步探讨菌群异常在鼻咽癌发生中的作用。方法以7例明确诊断且尚未治疗的鼻咽鳞癌患者和5例健康对照者为研究对象,提取鼻咽灌洗液DNA,观察两组研究对象菌群分布差异,进行相似性、多样性分析。结果鼻咽癌患者与健康对照者之间鼻咽灌洗液的菌群分布存在差异,与健康对照者比较,咽颊炎链球菌和不动杆菌数量增加,但嗜热链球菌数量减少。结论鼻咽癌患者鼻咽灌洗液菌群分布与健康对照者比较出现明显差异,可为鼻咽癌预防及治疗提供实验依据。Objective To explore the role of microbiota abnormality in pathogenesis of nasopharyngeal carcinoma.Methods With 5 healthy individuals as the controls and 7 patients diagnosed as nasopharyngeal squamous carcinoma who had not received any treatment as the subjects,polymerase chain reaction-modified gradient gel electrophoresis（PCR-DGGE）gene fingerprint technology was used to observe the differences in the microbiota in nasopharyngeal lavage fluid between the patients and healthy individuals.The total DNA was extracted from the nasopharyngeal lavage fluid.The similarity and diversity of microbiota between the two groups were analysed.Results There were significant differences in the distribution of the microbiota in nasopharyngeal lavage fluid between patients and healthy controls.Compared with the controls,the relative abundances of Streptococcus anginosus and Acinetobacters were significantly higher while that of Streptococcus thermophilus was lower in the patients.Conclusion There is significant difference in the distribution of microbiota in the nasopharyngeal lavage fluid between the patients and healthy controls.This study can provide experimental evidence for the prevention and treatment of nasopharyngeal carcinoma.

关 键 词：鼻咽癌 菌群 多样性 

分 类 号：R378[医药卫生—病原生物学]