当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的影响  

Effects of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82

作　　者：张艳霞[1] 蔺兴遥[1] 安方玉[1] 张正顺[2] 陈绍仪 周佳玮[1] 张延英[1] ZHANG Yanxia;LIN Xingyao;AN Fangyu;ZHANG Zhengshun;CHEN Shaoyi;ZHOU Jiawei;ZHANG Yanying(Gansu University of Traditional Chinese Medicine/ Key Laboratory of TCM Quality and Standard of Gansu Province, Lanzhou 730000, Gansu, China;Baiyin Branch of Gansu Province Hospital of Traditional Chinese Medicine, Baiyin 730900, Gansu , China;The Second People ＂s Hospital of Baiyin , Baiyin 730900, Gansu , China)

机构地区：[1]甘肃中医药大学/甘肃省中药质量与标准研究重点实验室培育基地 [2]甘肃省中医院白银分院 [3]白银市第二人民医院

出　　处：《中国临床药理学与治疗学》2018年第1期29-33,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：白银市科技计划项目(2013-2-76Y);甘肃中医学院中青年科研基金项目(2305016601)

摘　　要：目的:探讨当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的作用。方法:MTT比色法检测当归挥发油对GLC-82细胞的细胞毒作用;流式细胞术检测GLC-82细胞凋亡;吖啶橙/溴化乙锭(AO/BE)双荧光染色法观察GLC-82细胞凋亡。结果:当归挥发油在浓度6.25~200μg/m L范围内对GLC-82细胞的增殖具有浓度依赖性抑制作用,对GLC-82细胞的IC_(50)为113.03μg/m L。流式细胞术检测结果表明,当归挥发油可诱导GLC-82细胞凋亡、坏死,且细胞凋亡、坏死率随药物浓度增高而升高。AO/BE双荧光染色法结果显示,用药组随着药物浓度的升高,细胞结构固缩加重,凋亡、坏死细胞增多。结论:当归挥发油具有诱导GLC-82细胞凋亡的作用。AIM： To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell GLC-82. METHODS： MTT colorimetric assay was used to investigate the cytotoxic effect of human lung adenocarcinoma cell GLC-82. The cell apoptosis was analyzed by flow cytometry. AO/BE fluorescent staining was applied to observe the cell apoptosis. RESULTS： The GLC-82 cell was inhibited by volatile oil of the angelica in a concentration-dependent manner; the IC50 was 113. 03 μg/m L. The result of flow cytometry indicated volatile oil of the angelica could induce apoptosis and necrosis of GLC-82 cell,and the rates of apoptosis and necrosis were increased by the increase of drug concentration. The results of AO/BE fluorescent staining method indicated the rate of apoptosis and necrosis increased in drug group along with the increase of drug concentration. CONCLUSION： Volatile oil can induce the apoptosis of GLC-82 cell.

关 键 词：当归挥发油 人肺腺癌GLC-82细胞 细胞凋亡 

分 类 号：R965.2[医药卫生—药理学]