清肝泻火方对Graves病模型小鼠Th17细胞转录因子表达的影响  

Effects of Qinggan Xiehuo Prescription on Expression of Transcription Factors in Th17Cells of Graves' Disease Model Mice

作　　者：郭逸[1] 

机构地区：[1]湖北省中医院内分泌科,湖北武汉430070

出　　处：《山东中医杂志》2017年第9期795-797,803,共4页Shandong Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局"湖北省瘿病学术流派传承工作室"资助项目(国中医药办人教函[2012]170)

摘　　要：目的:观察清肝泻火方对Graves病(GD)模型小鼠Th17细胞相关转录因子的影响,从免疫反应角度研究清肝泻火方治疗GD的作用机制,探讨该方治疗GD的新靶点。方法:选取雌性BALB/c小鼠80只,其中60只以表达TSHR-A亚单位的重组腺病毒免疫制备GD小鼠模型,造模成功后按随机数字表法分为模型组、甲巯咪唑组和清肝泻火方组,每组各20只;剩余20只作为对照组。甲巯咪唑组每日灌服甲巯咪唑混悬液12 mg/kg,清肝泻火方组每日灌服清肝泻火方混悬液2 m L,对照组和模型组每日灌服蒸馏水2 m L,各组均连续干预30 d。采用放射免疫法(RIA)检测各组GD模型小鼠血清中游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平;酶联免疫吸附法检测各组GD模型小鼠血清中白细胞介素-17(IL-17)、白细胞介素-21(IL-21)水平。结果 :甲巯咪唑和清肝泻火方均可降低小鼠血清中FT3、FT4、TSH及IL-17、IL-21的表达水平,与模型组比较,差异有统计学意义(P<0.05)。结论:清肝泻火方能抑制Th17细胞相关转录因子的表达,从而抑制甲状腺自身的免疫炎症反应,促进GD小鼠甲状腺功能的恢复。Objective：To observe the effect of Qinggan Xiehuo prescription on the expression of transcription factors related to Thl7 cells of Graves＇disease（GD） model mice,investigate its mechanism in treating GD from the aspect of immune response and explore a new target in the treatment of GD with Qinggan Xiehuo prescription. Methods：A total of 80 female BALB/c mice were selected for the experiment. Among them,60 were chosen to construct the GD mouse model by using the recombinant adenovirus expressing the TSH re- ceptor A-subunit（TSHR-A）. After the models were established,by the random number table method,the 60 mice models were divided into three groups：the model group,the methimazole group and the Qinggan Xiehuo prescription group,with 20 mice in each group. The other 20 mice in the 80 served as the control group. Mice in the methimazole group were given methimazole suspension by gavage,12 mg/kg a day. Mice in the Qinggan Xiehuo prescription group were given Qinggan Xiehuo prescription suspension,2 mL a day. Mice in the control group and the model group were given distilled water,2 mL a day. All the mice were intervened for 30 days. The levels of serum free triiodothyronine（FT3）,free thyroxine（FT4） and thyroid stimulating hor mone（TSH） of mice in each group were measured by radioimmunoassay（RIA）. The levels of serum inter- leukin-17（IL-17） and interleukin-21（IL-21） of mice in each group were examined by enzyme-linked im- munosorbent assay（ELISA）. Results：Both methimazole and Qinggan Xiehuo prescription can reduce the ex- pression levels of serum FT3,FT4,TSH,IL-17 and IL-21. Compared with the model group,the differences were statistically significant（P〈0.05）. Conclusion：For the GD model mice,Qinggan Xiehuo prescription can inhibit the immune and inflammatory response of the thyroid gland and promote the reeovery of thyroidfunction by inhibiting the expression of transcription factors related to Thl7 cell.

关 键 词：清肝泻火方 GRAVES病 模型小鼠 Thl7细胞相关转录因子 

分 类 号：R285.5[医药卫生—中药学]