血管紧张素-(1-7)对血管紧张素Ⅱ诱导的子宫内膜上皮细胞增殖和转分化的影响  

Effect of angiotensin(-1-7) on proliferation and transdifferentiation of endometrial epithelium cells induced by angiotensin Ⅱ

作　　者：张文博[1] 单铁英[2] 韩文华[3] 王春漩 朱惠 杨以会[2] 潘秀兰[2] 任俊利 

机构地区：[1]邯郸市中心医院妇产科,河北邯郸056002 [2]河北工程大学医学院组胚教研室,河北邯郸056002 [3]河北工程大学附属医院内科,河北邯郸056002 [4]邯郸市中医院,河北邯郸056002 [5]武安市中医院内科,河北武安056300 [6]邯郸市中心医院儿科,河北邯郸056002

出　　处：《职业与健康》2017年第23期3227-3230,3236,共5页Occupation and Health

摘　　要：目的了解血管紧张素Ⅱ(AngⅡ)和血管紧张素-(1-7)[Ang-(1-7)]对子宫内膜上皮细胞(EECs)增殖和活性的影响。方法体外分离、培养和鉴定EECs。试验分4组:对照组、AngⅡ组、Ang-(1-7)组和AngⅡ+Ang-(1-7)组,采用噻唑蓝(MTT)法观察各组EECs增殖的影响;免疫细胞化学染色法检测各组EECs中的α-平滑肌肌动蛋白(α-SMA)和上皮性钙粘附素(E-cadherin)的表达水平;用酶联免疫吸附试验(ELISA)检测各组上清液中Ⅰ型胶原(ColⅠ)和纤粘连蛋白(FN)的含量。结果在48和72 h时,AngⅡ组EECs数量明显增加;细胞中α-SMA蛋白的表达升高(77.67±1.78),E-cadherin的表达降低(26.56±2.09);ColⅠ和FN分泌均增加(0.832 0±0.029 2,0.752 8±0.071 2),与对照组相比,其差异有统计学意义(P<0.05)。Ang-(1-7)+AngⅡ组的EECs数量减少;细胞胞质中α-SMA的含量降低(33.58±1.34),而E-cadherin的含量升高(66.86±2.76);ColⅠ和FN分泌均减少(0.635 5±0.026 5,0.557 3±0.038 5),与AngⅡ组相比,其差异有统计学意义(P<0.05)。结论 Ang-(1-7)不仅可拮抗AngⅡ刺激的EECs增殖,还可抑制其活性。[Objective]To understand the effect of angiotensinⅡ（AngⅡ）and angiotensin-（1-7）[Ang-（1-7）]on the proliferation and transdifferentiation of endometrial epithelium cells（EECs）. [Methods]The EECs was isolated,cultured and identified in vitro. The EECs was divided into four groups：control group,Ang Ⅱ group,Ang-（1-7） group and Ang Ⅱ ＋Ang-（1-7）group. The proliferation of EECs in four groups was observed by MTT mothed;the expression level of α-SMA and E-cadherin protein were detected by immunocytochemistry staining method;and the contents of Fibronectin（FN） and collagen type Ⅰ（Col Ⅰ） in the cultured supernatants were detected by ELISA.[Results] At 48 h and 72 h,the number of EECs in Ang Ⅱ group increased statistically;the expression of α-SMA protein in cells increased（77.67 ±1.78）,while the expression of E-cadherin decreased（26.56±2.09）;the secretion of ColⅠand FN increased（0.832 0±0.029 2,0.752 8±0.071 2）,and compared with the control group,the difference was statistically significant（P〈0.05）. The number of EECs in Ang-（1-7）＋Ang Ⅱ group decreased;the content of α-SMA in cytoplasm decreased（33.58±1.34）,while the content of E-cadherin increased（66.86±2.76）;the secretion of ColⅠand FN protein decreased（0.635 5±0.026 5,0.557 3±0.038 5）,and compared with the Ang-Ⅱgroup,the difference was statistically significant（P〈0.05）.[Conclusion]Ang-（1-7）could inhibit the proliferation of EECs induced by AngⅡand its viability.

关 键 词：血管紧张素-(1-7) 血管紧张素Ⅱ 子宫内膜上皮细胞 Α-平滑肌肌动蛋白 上皮性钙粘附素 Ⅰ型胶原 纤粘连蛋白 

分 类 号：R34[医药卫生—基础医学]