尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞葡萄糖消耗的影响  

Effects of Different Fractions from Gmtianella Acuta on the Glucose Consumption of H4ⅡE Cells with Insulin Resistance

作　　者：高佳琪[1,2,3] 魏颖 王东超[1,2,3] 石浩霞 李博[1,2,3] 秦灵灵 孙文 兰卫[4] 徐暾海 刘铜华[2,3] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学中医养生学北京市重点实验室,北京100029 [3]北京中医药大学中医养生学教育部重点实验室,北京100029 [4]新疆医科大学,新疆乌鲁木齐830011

出　　处：《山东中医药大学学报》2017年第5期462-466,共5页Journal of Shandong University of Traditional Chinese Medicine

基　　金：基金项目 国家重大新药创制(编号:1000071320025)

摘　　要：目的:研究尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞糖消耗的影响。方法:采用CCK-8法评价H4ⅡE细胞活性;高浓度胰岛素(10-6mol/L)诱导培养H4ⅡE细胞36 h,建立新的胰岛素抵抗细胞模型。葡萄糖氧化酶法检测尖叶假龙胆不同提取部位对胰岛素抵抗H4ⅡE细胞糖消耗的影响。结果:尖叶假龙胆不同提取部位浓度大于150μg/m L时,对H4ⅡE细胞活性均有明显的抑制作用。尖叶假龙胆不同提取部位中乙酸乙酯提取部位对胰岛素抵抗H4ⅡE细胞葡萄糖消耗影响显著,大孔树脂95%提取部位与大孔树脂70%提取部位也不同程度地影响胰岛素抵抗H4ⅡE细胞葡萄糖消耗,大孔树脂30%提取部位效果不明显。结论:尖叶假龙胆乙酸乙酯提取部位改善胰岛素抵抗效果最佳,大孔树脂95%提取部位与大孔树脂70%提取部位也可不同程度地改善胰岛素抵抗作用。Objective：To study the effects of different fractions from Gmtianella acuta influence on the glucose consumption of H4 Ⅱ E cells with insulin resistance.Methods：The H4 Ⅱ E cell activity was evaluated with method for the CCK-8.The H4ⅡE cells was cultured about 36 hours by insulin of high concentration（10-6mol/L） to establish new insulin resistance cell model.The effects of different fractions from Gmtianella acuta on the glucose consumption of H4ⅡE cells with insulin resistance was tested by glucose oxidase.Results：The effects of different fractions from Gmtianella acuta on H4 Ⅱ E cell activity were significantly inhibited,when their concentrations greater than 150 μg/m L.The ethyl acetate fraction of Gmtianella acuta significantly affected the glucose consumption of H4 Ⅱ E cells with insulin resistance.Macroporous resin-95% fraction and macroporous resin-70% fraction affected the glucose consumption of H4ⅡE cells with insulin resistance with different degrees,while macroporous resin-30% had no significant effects.Conclusion：The ethyl acetate fraction of Gmtianella acuta had best effects on insulin resistance,macroporous resin-95% fraction and macroporous resin-70% fraction could improve the insulin resistance with different degrees.

关 键 词：尖叶假龙胆 H4ⅡE细胞 胰岛素抵抗 葡萄糖消耗 

分 类 号：R285.5[医药卫生—中药学]